Increase in Iron and Zinc Concentrations in Rice Grains Via the Introduction of Barley Genes Involved in Phytosiderophore Synthesis

By H. Masuda, Motofumi Suzuki, Kendi Claudio Morikawa, Takanori Kobayashi, H. Nakanishi, Michiko Takahashi, M. Saigusa, S. Mori, N. Nishizawa. Rice, 2008

138 citations recorded by the source corpus.

Increasing the iron (Fe) and zinc (Zn) concentrations of staple foods, such as rice, could solve Fe and Zn deficiencies, which are two of the most serious nutritional problems affecting humans. Mugineic acid family phytosiderophores (MAs) play a very important role in the uptake of Fe from the soil and Fe transport within the plant in graminaceous plants. To explore the possibility of MAs increasing the Fe concentration in grains, we cultivated three transgenic rice lines possessing barley genome fragments containing genes for MAs synthesis (i.e., HvNAS1, HvNAS1, and HvNAAT-A and HvNAAT-B or IDS3) in a paddy field with Andosol soils. Polished rice seeds with IDS3 inserts had up to 1.40 and 1.35 times higher Fe and Zn concentrations, respectively, compared to non-transgenic rice seeds. Enhanced MAs production due to the introduced barley genes is suggested to be effective for increasing Fe and Zn concentrations in rice grains.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/a025e47402b0e3119e8b706ecbe20867cdf1e13e

How researchers see this paper

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In Plant Science

1. N. Nishizawa
2. G. R. Rout
3. S. Sahoo
4. Yasuhiro Ishimaru
5. Khurram Bashir
6. Takanori Kobayashi
7. H. Nakanishi

Citations in the corpus

• Zn Uptake and Translocation in Rice Plants (2011, background): “However, the seed Fe and Zn concentrations did not increase in plants grown under Fe-deficient conditions in calcareous paddy fields or under Fe-sufficient conditions in andosol paddy fields (Masuda et al. 2008; Suzuki et al. 2008a).”
• Overexpression of the Barley Nicotianamine Synthase Gene HvNAS1 Increases Iron and Zinc Concentrations in Rice Grains (2009, background): “However, Fe and Zn concentrations did not increase in seeds collected from HvNAS1 genome insertion rice plants when grown under Fe-deficient conditions in calcareous paddy fields or under Fe-sufficient conditions in andosol paddy fields (Masuda et al. 2008; Suzuki et al. 2008a).”
• Iron uptake, translocation, and regulation in higher plants. (2012, background): “Field trials have revealed that a rice line carrying a barley IDS3 genome fragment exhibits both Fe deficiency tolerance in calcareous soils and moderately increased Fe and Zn concentration in its grains in both calcareous and Andosol paddy fields (71, 119).”
• ROLE OF IRON IN PLANT GROWTH AND METABOLISM (2015, methodology): “The iron concentrations in the seeds of transgenic lines were analyzed after cultivation in the paddy field in iron-sufficient or low (calcareous) soil (Masuda et al., 2008; Suzuki et al., 2008).”