Overexpression of the Barley Nicotianamine Synthase Gene HvNAS1 Increases Iron and Zinc Concentrations in Rice Grains

By H. Masuda, Kanako Usuda, Takanori Kobayashi, Yasuhiro Ishimaru, Yusuke Kakei, Michiko Takahashi, K. Higuchi, H. Nakanishi, S. Mori, N. Nishizawa. Rice, 2009

203 citations recorded by the source corpus.

In humans, iron (Fe) and zinc (Zn) deficiencies result in major worldwide health problems. Transgenic technologies to produce Fe- and Zn-biofortified rice varieties offer a promising potential solution. Nicotianamine, the precursor of phytosiderophores, chelates Fe2+ and Zn2+ and plays an important role in transporting these metals to both vegetative and reproductive organs within the plant. Our objective was to increase Fe and Zn contents in rice grains by overexpressing the barley nicotianamine synthase gene HvNAS1. HvNAS1-overexpressing transgenic rice showed increased HvNAS1 expression and subsequent increases in endogenous nicotianamine and phytosiderophore content in shoots, roots, and seeds. Fe and Zn concentrations in polished T1 seeds from transgenic plants increased more than three and twofold, respectively; Fe and Zn concentrations also increased in both polished and brown T2 seeds. These results suggest that the overproduction of nicotianamine enhances the translocation of Fe and Zn into rice grains.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/59f5eeff946b1900d58c2bcac3c6a03debed57b4

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Citations in the corpus

Iron uptake, translocation, and regulation in higher plants. (2012, background): “Also, overexpression of the NAS genes HvNAS1 or OsNAS3 in rice is effective at increasing Fe and Zn up to threeand twofold, respectively, in their grains (60, 72).”
Zn Uptake and Translocation in Rice Plants (2011, background): “Rice seeds contain not only Zn but also NA and DMA, and the amount of DMA is significantly higher than that of NA (Usuda et al. 2009; Masuda et al. 2009).”
Influence of Soil Chemistry and Plant Physiology in the Phytoremediation of Cu, Mn, and Zn (2014, background): “The overexpression of the barley NA synthase HvNAS1 in rice showed increases in endogenous phytosiderophore content in shoots, roots, and seeds, resulting in higher Zn levels in rice seeds (Masuda et al., 2009).”
Siderophores in environmental research: roles and applications (2014, background): “which produce a lower concentration of phytosiderophores (e.g. Masuda et al., 2009; Kobayashi et al., 2010).”
Iron Uptake and Loading into Rice Grains (2010, background): “Rice transformed with barley IDS3 (a mugineic acid-synthesizing dioxygenase gene) accumulated more Fe in the grain (Masuda et al. 2008).”