U6 promoter–driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells

By M. Miyagishi, K. Taira. Nature Biotechnology, 2002

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siRNA vs. shRNA: similarities and differences. (2009, background): “be active and it was soon demonstrated that active siRNA consists of a hairpin structure can be transcribed in cells from an RNA polymerase III promoter on a plasmid construct [49,50].”
Killing the messenger: short RNAs that silence gene expression (2003, background): “Although the rules that govern efficient siRNA-directed gene silencing remain undefined ( Box 1 ), it is known that siRNAs that target different regions of the same gene vary markedly in their effectivenes”
Cloning of adiponectin receptors that mediate antidiabetic metabolic effects (2003, background): “Expression of AdipoR1/R2 or suppression of AdipoR1/R2 expression by small-interfering RN”
RNAi therapeutics: principles, prospects and challenges. (2007, background): “The separately expressed strands hybridize and produce functional siRNAs [34,36].”
RNA silencing: Methods and protocols (2006, methodology): “Bloodstream trypanosomes expressing T7 polymerase and the tet repressor, which are not resistant to hygromycin, such as the “single-marker” line containing pHD 328 and pLew114hyg5′ (25).”
RNAi suppresses polyglutamine-induced neurodegeneration in a model of spinocerebellar ataxia (2004, methodology): “Blots were probed with 32 P-labeled sense oligonucleotides at 36 °C for 3 h to detect transcripts, washed and exposed to film overnigh”