Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk

By K. Hunt, James A. Foster, Larry J. Forney, Ursel M. E. Schütte, Daniel Beck, Z. Abdo, L. Fox, Janet E. Williams, M. McGuire, Mark A. McGuire. PLoS ONE, 2011

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Recent investigations have demonstrated that human milk contains a variety of bacterial genera; however, as of yet very little work has been done to characterize the full diversity of these milk bacterial communities and their relative stability over time. To more thoroughly investigate the human milk microbiome, we utilized microbial identification techniques based on pyrosequencing of the 16S ribosomal RNA gene. Specifically, we characterized the bacterial communities present in milk samples collected from 16 women at three time-points over four weeks. Results indicated that milk bacterial communities were generally complex; several genera represented greater than 5% of the relative community abundance, and the community was often, yet not always, stable over time within an individual. These results support the conclusion that human milk, which is recommended as the optimal nutrition source for almost all healthy infants, contains a collection of bacteria more diverse than previously reported. This finding begs the question as to what role this community plays in colonization of the infant gastrointestinal tract and maintaining mammary health.

Subfields: Epidemiology, Molecular Biology, Nutrition and Dietetics

View Paper: https://www.semanticscholar.org/paper/9d8080e730fc3ba18590e371ca5f51382d0ed7cb

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Citations in the corpus

Mom Knows Best: The Universality of Maternal Microbial Transmission (2013, background): “nine genera present in every sample: Staphylococcus, Streptococcus, Serratia, Pseudomonas, Corynebacterium, Ralstonia, Propionibacterium, Sphingomonas, and Bradyrhizobiaceae [78].”
The human milk microbiome changes over lactation and is shaped by maternal weight and mode of delivery. (2012, background): “The higher amount of diversity and the different bacterial composition found in our data sets when compared with the other available data (see Figure S1 under “Supplemental data” in the online issue) could be a result of geographic and environmental differences in the mothers and secondly of differe”
The human milk microbiota: origin and potential roles in health and disease. (2013, background): “[25]”
The complex microbiota of raw milk. (2013, background): “…derived from a number of separate studies (Heikkila & Saris, 2003; Delbes et al., 2007; Devirgiliis et al., 2008; Maniruzzaman et al., 2010; Hunt et al., 2011; Masoud et al., 2011; Alegria et al., 2012; Cabrera-Rubio et al., 2012; Ercolini et al., 2012; Quigley et al., 2013) using…”
Variation in Microbiome LPS Immunogenicity Contributes to Autoimmunity in Humans. (2016, neutral): “In vaginal births, most of the early infant gut colonizers are derived from the mother’s gut (Bäckhed et al., 2015) and possibly consolidated by the microbiome in breast milk (Hunt et al., 2011).”
The healthy human microbiome (2016, background): “Likewise, breast milk [41] and the placenta [42] are of interest for the early establishment of both a healthy microbiome and the potential circulating blood [43] or tissue [44, 45] microbiomes for normal immune control of opportunists.”
The infant microbiome development: mom matters (2014, neutral): “The breast-milk microbiota is dominated by a few genera (Staphylococcus, Streptococcus, Serratia, Pseudomonas, Corynebacterium, Ralstonia, Propionibacterium, Sphingomonas, and Bradyrhizobiaceae) [55].”
Human milk oligosaccharides: every baby needs a sugar mama. (2012, background): “Human milk per se is not sterile and contains complex bacterial communities that are often highly personalized to the lactating woman (Martin et al. 2007; Hunt et al. 2011).”
The composition of the gut microbiota throughout life, with an emphasis on early life (2015, background): “Breast milk also contains an abundance of complex oligosaccharides with prebiotic activity, stimulating the growth of specific bacterial groups such as staphylococci (47) and bifidobacteria (49).”