Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5

By X. Qin, D. An, I. Chen, David Baltimore. Proceedings of the National Academy of Sciences of the United States of America, 2002

740 citations recorded by the source corpus.

How researchers see this paper

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Killing the messenger: short RNAs that silence gene expression (2003, background): “To overcome some of these limitations, several groups have reported the use of retrovirus vectors to deliver siRNAs into cell”
SIRT2 deacetylates FOXO3a in response to oxidative stress and caloric restriction (2007, methodology): “NIH3T3 cells with SIRT2 shRNA knock-down were generated using a lentiviral-mediated delivery system as reported (Qin et al., 2003).”
RNAi therapeutics: principles, prospects and challenges. (2007, methodology): “[103] used a lentiviral vector to transduce a Pol III expressed anti-CCR5 shRNA in human lymphocytes.”
Conditional Suppression of Cellular Genes: Lentivirus Vector-Mediated Drug-Inducible RNA Interference (2003, background): “Recently, it was demonstrated that the knockdown of endogenous genes could be achieved by RNA interference, and plasmid- or viral vector-based delivery systems for the stable expression of small interfering RNAs (siRNAs) were rapidly created (1, 3, 5, 7, 9, 17).”
Jurkat T cells and development of the T-cell receptor signalling paradigm (2004, background): “Nonetheless, many laboratories are now applying siRNAs to various lymphoid cell lines, and it is most encouraging to learn that siRNA technology can be used to silence genes in normal human T cell”