Spheroid-based drug screen: considerations and practical approach

By J. Friedrich, C. Seidel, R. Ebner, Leoni A. Kunz-Schughart. Nature Protocols, 2009

1,618 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Cell Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/995d809b7cb51dc28aefb034b9bf2bd20637e242

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Citations in the corpus

Pharmacological inhibition of cystine–glutamate exchange induces endoplasmic reticulum stress and ferroptosis (2014, background): “DOI: 10.7554/eLife.02523 2 of 25 Research article unsuccessful (Robe et al., 2009).”
Cholangiocarcinoma 2020: the next horizon in mechanisms and management (2020, positive): “To recapitulate more adequately the in vivo tumour tissue structure and to investigate the interaction between CCA and the TME, 3D model systems, known as tumour spheroids and organoids, were developed 240,241 .”
Size-Dependent Localization and Penetration of Ultrasmall Gold Nanoparticles in Cancer Cells, Multicellular Spheroids, and Tumors in Vivo (2012, neutral): “MCF-7 spheroids were produced by the liquid overlay method as previously described.(49) Briefly, cells were detached from monolayers, and single cell suspensions (200 μL per well containing 600 cells) were transferred into flat-bottomed 96-well plates precoated with 1% agarose.”
Spherical Cancer Models in Tumor Biology (2015, background): “Experts in the field recommend their use in major programs for drug screening and development and frequently point out the rationale for using MCTSs in antitumor drug testing [49] (Table 3).”
Stimuli-responsive clustered nanoparticles for improved tumor penetration and therapeutic efficacy (2016, background): “Compared with single-layer adherent cells, MCSs have been proposed as a versatile 3D model to study tumor biology and to screen therapeutic agents (41).”
Multicellular tumor spheroids: an underestimated tool is catching up again. (2010, background): “, 1997) or cellular aggregation by sedimentation at concave and cell adhesion resistant surfaces, such as agarose (Friedrich et al., 2009), poly-hydroxyethyl methacrylate (poly-HEMA) (Ivascu and Kubbies, 2006) or a droplet’s air–liquid interface (Kelm and Fussenegger, 2004; Timmins and Nielsen, 2007”
Opportunities and Challenges for use of Tumor Spheroids as Models to Test Drug Delivery and Efficacy (2012, neutral): “Testing in multicellular spheroids is increasingly regarded as an essential step in drug development [16, 43, 134, 144, 145].”
Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning (2018, background): “Low adhesion plates are often made of polystyrene and treated with hydrophilic or hydrophobic coatings like the non-adherent polymer poly-HEMA (Ivascu and Kubbies, 2006) or natural polymers such as agarose (Friedrich et al., 2009; Li et al., 2011).”
In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform (2016, background): “…on recapitulating solid tumors and studying growth kinetics (size versus time), composition, and tumor cell biology (e.g., proliferation, differentiation, cell death, protein and gene expression, etc.) (Sutherland et al., 1971; Freyer and Sutherland, 1986; Durand, 1990; Friedrich et al., 2009).”