Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors

By L. Foster, C. L. de Hoog, Matthias Mann. Proceedings of the National Academy of Sciences of the United States of America, 2003

868 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/99594f6d49ea6338097526d40aa3f78154217562

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Citations in the corpus

Lipid rafts in T cell receptor signalling (Review) (2006, methodology): “Proteomic analysis has shown the presence of protein contaminants in DRM preparations [23-25], therefore in order to piece together a more complete picture of how lipid rafts function in T cells, other methods should be applied as well.”
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (2006, methodology): “Protein digestion was performed essentially as in Foster et al. (2003), strong-cation exchange chromatography of digests as in Gruhler et al. (2005), and TiO2 enrichment of phosphopeptides essentially as in Larsen et al. (2005).”
Comparative evaluation of mass spectrometry platforms used in large-scale proteomics investigations (2005, background): “, with many studies identifying proteins numbering in the thousand”
The Calcium Channel α2δ-2 Subunit Partitions with CaV2.1 into Lipid Rafts in Cerebellum: Implications for Localization and Function (2006, background): “These proteins are submembrane scaffold proteins that are known constituents of lipid rafts in both the plasma membrane and endocytic vesicles (Foster et al., 2003; Langhorst et al., 2005; Morrow and Parton, 2005; Rivera-Milla et al., 2006).”
SYMPOSIUM REVIEW: Lipid microdomains and the regulation of ion channel function (2010, neutral): “While this method suffers from none of the potential selective extraction that plagues detergent-based methods, there is evidence that contaminating non-raft proteins may stay associated at the periphery of rafts and float with them to the low density layers (Foster et al. 2003; Pike, 2004).”
The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins (2006, methodology): “In-solution digestion using urea was done essentially as described previously by Foster and coworkers [ 61 ].”
The Protein Composition of Mitotic Chromosomes Determined Using Multiclassifier Combinatorial Proteomics (2010, neutral): “Genuine components have been distinguished from contaminants in such preparations by subtractive (Schirmer et al., 2003) or quantitative (Foster et al., 2003) proteomics by determining the difference between two near-identical fractions, one enriched and the other depleted of thetargetstructure.”
Parts per Million Mass Accuracy on an Orbitrap Mass Spectrometer via Lock Mass Injection into a C-trap*S (2005, methodology): “In-solution Digestion of BSA—One mg of lyophilized BSA (SigmaAldrich) was resolubilized in a buffer containing 6 M urea (Invitrogen), 2 M thiourea (Fluka), and reduced, alkylated, and digested essentially as described (15).”
Lipid Rafts and Caveolae as Portals for Endocytosis: New Insights and Common Mechanisms (2003, background): “This has been used to great effect to determine whether proteins are bona fide lipid raft components or merely copurified contaminants (26), as well as to determine the role of rafts in cellular processes (27).”
Upregulated function of mitochondria-associated ER membranes in Alzheimer disease (2012, neutral): “It may also explain why MAM markers such as VDAC (Szabadkai et al , 2006) and calnexin (Myhill et al , 2008) were found in some studies of PM rafts (Foster et al , 2003; Zheng et al , 2009) and why A b was reported to be present in mitochondria (Lustbader et al , 2004; Manczak et al , 2006).”
Exponentially Modified Protein Abundance Index (emPAI) for Estimation of Absolute Protein Amount in Proteomics by the Number of Sequenced Peptides per Protein*S (2005, methodology): “studies between two states (5, 6) but also protein-protein (7, 8), protein-peptide (9), and protein-drug (10) interaction analyses have been reported.”