MethPrimer: designing primers for methylation PCRs

By Long-Cheng Li, Rajvir Dahiya. Bioinform., 2002

2,624 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/97a133cb22e487bb471945f6e62aa5b087d80b00

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In Molecular Biology

Citations in the corpus

PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR (2004, methodology): “Unlike an alternative program, Methprimer (Li and Dahiya, 2002), PerlPrimer by default selects for primers with converted cytosine bases at the 3 end—allowing specific amplification of bisulphiteconverted templates.”
BiQ Analyzer: visualization and quality control for DNA methylation data from bisulfite sequencing (2005, background): “On the one hand, several primer design websites (Li and Dahiya, 2002; Tusnady et al., 2005) help the experimenter to prepare DNA methylation experiments, a problem that is upstream of the data processing task that we consider here.”
Epigenetic gene promoter methylation at birth is associated with child’s later adiposity (2011, methodology): “amplification of bisulfite-treated DNA was followed by in vitro transcription and analysis by matrix-assisted laser desorption ionization time-of-flight (MALDITOF) mass spectrometry (27,28).”
A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening (2006, methodology): “Forward (F) and reverse (R) primers ( Supplementary Table 2 online) for bisulfite-sequencing PCR were designed to the forward strand of the likely LeSPL-CNR promoter using MethPrimer softwar”
EPIGENETIC REGULATION OF BDNF GENE TRANSCRIPTION IN THE CONSOLIDATION OF FEAR MEMORY (2008, methodology): “It is important to note that in a circumstance in which a specific promoter is in a highly methylated or unmethylated basal state, the two different primer sets will be preferentially sensitive to alterations in methylation (Li and Dahiya, 2002).”
DNA methylation analysis by pyrosequencing (2007, methodology): “For the design of amplification primers, several software tools are available free of charge on the internet such as MethPrime”
CREB/ATF-dependent T cell receptor–induced FoxP3 gene expression: a role for DNA methylation (2007, methodology): “Strikingly, analysis of the FoxP3 genomic locus with the MethPrimer computer program (24) revealed a single putative CpG island in the +4,393 to +4,506 region (Fig.”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, positive): “Bisulphite-specific primers were based on the returned, bisulphite-specific sequence from MethPrimer software (Li and Dahiya, 2002).”
Cytomegalovirus Infection Drives Adaptive Epigenetic Diversification of NK Cells with Altered Signaling and Effector Function (2015, neutral): “Primers were designed using the MethPrimer program (Li and Dahiya, 2002).”