DNA methylation analysis by pyrosequencing

By Jorg Tost, Ivo G. Gut. Nature Protocols, 2007

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Epigenetic transmission of the impact of early stress across generations. (2010, result): “01; sperm, n 4 –5; brain, ontrol (n 8) brain [t(14) 2.”
Genome-wide methylation analysis of human colon cancer reveals similar hypo- and hypermethylation at conserved tissue-specific CpG island shores (2009, neutral): “Bisulfite pyrosequencing was done as previously describe”
A Primer on Metagenomics (2010, background): “Pyrosequencing already offers a capability for quantitative methylation [130] and in all likelihood methylation data will be soon made available routinely along with the four base data, and the associated bioinformatics would need to address that.”