Improved plant-based production of E1 endoglucanase using potato: expression optimization and tissue targeting

By Z. Dai, B. Hooker, Daniel B. Anderson, Steven R. Thomas. Molecular breeding, 2000

98 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize plants (2006, background): “Production of E1 has been previously reported in tobacco [9], Arabidopsis [8], potato [10], and barley [11].”
Commercial cellulosic ethanol: The role of plant-expressed enzymes (2009, background): “Subsequently, A. cellulolyticus endoglucanase E1 was found in truncated but active form in tobacco and in duckweed, consistent with proteolytic removal of the cellulose binding domain (CBD), leaving just the catalytic domain (CD; Dai et al. 2000a; Sun et al. 2007).”
Dramatic effects of truncation and sub-cellular targeting on the accumulation of recombinant microbial cellulase in tobacco (2001, background): “A similar finding was reported by Dai et al. (2000b) for transgenic potato plants expressing apoplast- and chloroplast-localized E1.”
Ethanol fermentation from biomass resources: current state and prospects (2006, background): “The thermostable endoglucanase E1 from Acidothermus cellulolyticus was expressed in Arabidopsis thaliana leaves (Ziegler et al. 2000), potato ( Dai et al. 2000 ), and tobacco (Hooker et al. 2001).”