Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize plants

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Commercial cellulosic ethanol: The role of plant-expressed enzymes (2009, background): “A. cellulolyticus EG has been expressed as high as 2% total soluble protein (TSP) in corn stover (Biswas et al. 2006; Mei et al. 2009), but it is estimated that 10% TSP is needed for complete hydrolysis (Sticklen 2008).”
Genetic modification of lignin biosynthesis for improved biofuel production (2009, background): “…of cellulose and hemicellulose pathway enzymes or other enzymes involved in increasing plant biomass characteristics or production of recombinant cellulases or hemicellulases in plants (Ziegelhoffer et al. 1999; Ericksson et al. 2000; Biswas et al. 2006; Oraby et al. 2007; Ransom et al. 2007).”
Advancing environmental risk assessment for transgenic biofeedstock crops (2009, background): “Highly thermostable forms of alpha-amylase and endoglucanase have been expressed in maize kernel starch endosperm [50], ubiquitously in roots and shoots [45] or in green tissues only [48] to affect improved economies and efficiencies of ethanol production.”