Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

By M. Matano, Shoichi Date, M. Shimokawa, Ai Takano, Masayuki Fujii, Y. Ohta, Toshiaki Watanabe, T. Kanai, Toshiro Sato. Nature Medicine, 2015

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Delivering CRISPR: a review of the challenges and approaches (2018, methodology): “In other examples, electroporation was used to deliver plasmid DNA encoding both Cas9 and mRNA to generate colorectal cancer models from Cas9-engineered human intestinal organoids (Matano et al., 2015), generate an early-onset Alzheimer’s disease model in human DRUG DELIVERY 1243”
Stem Cell Organoid Engineering (2016, positive): “introduced multiple mutations in niche signaling pathways into human intestinal epithelial organoids, which were cultured in vitro and then transplanted into mice so their role in tumor progression and micrometastases could be studied (Matano et al., 2015).”
Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects (2020, methodology): “).(83) Moreover, guided by colonoscopy, through mucosal injection, Roper et al.”
Modeling Development and Disease with Organoids. (2016, background): “Xenotransplantation revealed the progressive transformation of the wild-type stem cells into adenocarcinomas (Drost et al., 2015; Matano et al., 2015).”