Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics.
By T. Kitamura, Yuko Koshino, F. Shibata, T. Oki, H. Nakajima, T. Nosaka, H. Kumagai. Experimental Hematology, 2003
756 citations recorded by the source corpus.
Subfields: Genetics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/9105306e1d4dcaf3a0f35f1c976b50c180813199
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In Genetics
In Molecular Biology
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Citations in the corpus
- PINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy (2010, methodology): “S1 A); consequently, HA or GFPParkin was introduced into these cells by retroviral transfection (Kitamura et al., 2003).”
- The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. (2012, positive): “Stable cell lines were generated using a retroviral expression system as previously described (Hara et al., 2008; Kitamura et al., 2003 Cells were placed on a glass bottom dish”
- Persistent activation of Nrf2 through p62 in hepatocellular carcinoma cells (2011, methodology): “Wild-type p62 and p62 T350A mutant were subcloned into retrovirus vector, pMXs-puro (Kitamura et al., 2003).”
- Hippo pathway regulation by cell morphology and stress fibers (2011, methodology): “Enhanced green fluorescent protein (EGFP)-expressing NIH3T3 cells were generated by infection with the retroviral vector pMYs-IRES-EGFP (Kitamura et al., 2003).”
- Discovery of the molecular machinery CERT for endoplasmic reticulum-to-Golgi trafficking of ceramide (2006, methodology): “Thus, we decided to employ retroviral vector-mediated transfection, which can introduce non-viral genes into mitotic cells stably with very high efficiency [26].”
- Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors (2010, methodology): “See the Extended Experimental Procedures for additional statistical methods.”
- Innate immune detection of the type III secretion apparatus through the NLRC4 inflammasome (2010, methodology): “Bacterial genes were cloned into pMXsIG (32), which contains an IRES-GFP element to track retroviral infection.”