Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells

By M. Richards, C. Fong, W. Chan, P. Wong, A. Bongso. Nature Biotechnology, 2002

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Human embryonic stem cells express an immunogenic nonhuman sialic acid (2005, methodology): “We therefore incubated HESC in medium containing heat-inactivated human serum instead of the standard serum replacement (an approach already suggested by others for different reasons”
Embryonic stem cells: prospects for developmental biology and cell therapy. (2005, background): “The resulting hES cells shared some fundamental characteristics of murine lines, such as Oct-3/4 expression, telomerase activity, and the formation of teratomas containing derivatives of all three primary germ layers in immunodeficient mice (295, 362).”
Feeder Layer- and Serum-Free Culture of Human Embryonic Stem Cells1 (2004, background): “In recent years, extensive investigation into improving culture systems for hES cells has yielded three main advances: 1) the ability to grow cells under serum-free conditions [1]; 2) the maintenance of the cells in an undifferentiated state on Matrigel matrix with 100% mouse embryonic fibroblast (M”