Polymorphism and Discrimination Capacity of Randomly Amplified Polymorphic Markers in an Olive Germplasm Bank

By A. Belaj, I. Trujillo, Raúl de la Rosa, L. Rallo, M. Giménez. 2001

179 citations recorded by the source corpus.

Random amplified polymorphic DNA (RAPD) analysis was performed on the main Mediterranean cultivars of olive (Olea europaea L.) from the Germplasm Bank of the Centro de Investigacion y Formacion Agraria "Alameda del Obispo" in Cordoba, Spain. One hundred and ninety reproducible amplification fragments were identified using 46 random primers followed by agarose gel electrophoresis. Some 63.2% of the amplification products were polymorphic, with an average of 2.6 RAPD markers obtained for each primer. The combination of polymorphic markers resulted in 244 banding patterns. The high degree of polymorphism detected made identification of all the cultivars (51) possible by combining the RAPD banding patterns of just only four primers: OPA-01, OPK-08, OPX-01, and OPX-03. Cultivar- specific RAPD markers and banding patterns were also found. A dendrogram based on unweighted pair-group method cluster analysis was constructed using a similarity matrix derived from the RAPD amplification products generated by the 46 primers. Three major groups of cultivars could be distinguished by RAPD analysis: 1) cultivars from east and northeast Spain, 2) Turkish, Syrian, and Tunisian cultivars, and 3) the majority of common olive cultivars in Spain. The dendrogram thus showed a good correlation between the banding patterns of olive cultivars and their geographic origin. A higher level of polymorphism was observed when polyacrylamide gel electrophoresis was used to separate the amplification products. Thus, adequate use of RAPD technology offers a valuable tool to distinguish between olive cultivars.

Subfields: Genetics, Molecular Biology, Organic Chemistry

View Paper: https://www.semanticscholar.org/paper/8ee1ddb04263cec5eaf25d2d0fda4e2a18698932

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Citations in the corpus

Self-incompatibility of `Arbequina' and `Picual' Olive Assessed by SSR Markers (2006, background): “Furthermore, it has been demonstrated using morphological (Barranco, 1997; Barranco and Rallo, 1984) and DNA markers (Belaj et al., 2001, 2004; Mekuria et al., 1999; Wiesman et al., 1998) that generic names such as ‘Gordal’, ‘Lechín’, ‘Manzanilla’, ‘Verdial’, etc., include different cultivars.”
Morphological and molecular analyses for the characterization of a group of Italian olive cultivars (2003, unknown): “, RAPDs (Wiesman et al., 1998; Mekuria et al., 1999; Belaj et al., 2000; Gemas et al., 2000; Besnard et al., 2001), microsatellites (Sefc et al., 2000; Carriero et al., 2002; Cipriani et al., 2002), mitochondrial DNA RFLPs (Besnard and Berville, 2000), multiple markers (Hess et al., 2000), isozymes ”
Genetic diversity among Jatropha species as revealed by RAPD markers (2008, methodology): “Among these, RAPD is an inexpensive and rapid method not requiring any information regarding the genome of the plant, and has been widely used to ascertain the genetic diversity in several plants (Belaj et al. 2001; Deshwall et al. 2005).”
Phenological growth stages of olive trees (Olea europaea) (2002, background): “Recently, identification of cultivars using RAPD molecular markers was made by Bogani et al. (1994), Fabbri et al. (1995), Vergari et al. (1996), Sanz-Cortes et al. (2001) and Belaj et al. (2001).”
Plastid and nuclear DNA polymorphism reveals historical processes of isolation and reticulation in the olive tree complex (Olea europaea) (2007, background): “The hypothesis of a continuous enrichment of olive trees with multiple wild Mediterranean genomes in the course of the domestication is compatible with the molecular similarity of wild and cultivated olives from the same country using multiple markers (Belaj et al., 2001; Besnard et al., 2001a; Varg”
Cultivar identification and genetic fingerprinting of temperate fruit tree species using DNA markers (2002, background): “Belaj et al. (2001) identified 51 olive accessions obtained in diverse countries with 46 RAPD primers,”
Genetic diversity in the Olive tree (Olea europaea L. subsp. europaea) cultivated in Portugal revealed by RAPD and ISSR markers (2004, methodology): “These plants are maintained by three man et al. 1998; Mekuria et al. 1999; Belaj et al. 2001; different methods: in a germplasm bank, in field trials ´Besnard et al. 2001b; Sanz-Cortes et al. 2001).”