Engineering hybrid exosomes by membrane fusion with liposomes
By Yuko T. Sato, Kaori Umezaki, S. Sawada, S. Mukai, Y. Sasaki, N. Harada, H. Shiku, K. Akiyoshi. Scientific Reports, 2016
679 citations recorded by the source corpus.
Exosomes are a valuable biomaterial for the development of novel nanocarriers as functionally advanced drug delivery systems. To control and modify the performance of exosomal nanocarriers, we developed hybrid exosomes by fusing their membranes with liposomes using the freeze–thaw method. Exosomes embedded with a specific membrane protein isolated from genetically modified cells were fused with various liposomes, confirming that membrane engineering methods can be combined with genetic modification techniques. Cellular uptake studies performed using the hybrid exosomes revealed that the interactions between the developed exosomes and cells could be modified by changing the lipid composition or the properties of the exogenous lipids. These results suggest that the membrane-engineering approach reported here offers a new strategy for developing rationally designed exosomes as hybrid nanocarriers for use in advanced drug delivery systems.
View Paper: https://www.semanticscholar.org/paper/8eb100973e79f17f28b958f8c84527f8b81e1e8f
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Citations in the corpus
- Exosome Theranostics: Biology and Translational Medicine (2018, background): “For example, a novel study that developed hybrid exosomes by fusing exosomes with liposomes required aggressive freeze-thaw processes (Figure 4) [190].”
- Advances in therapeutic applications of extracellular vesicles (2019, background): “Fusing EVs with synthetic liposomes modifies and tunes the exosomal interface to decrease immunogenicity, increase colloidal stability, and improve the half-life in circulation (146).”
- Exosome: A Review of Its Classification, Isolation Techniques, Storage, Diagnostic and Targeted Therapy Applications (2020, unknown): “132,133 The drug loading capacity of the freeze-thaw cycle is usually lower than that of sonication and extrusion.”