Evaluation of the nutritive value of Leucaena leucocephala leaf meal, inoculated with fish intestinal bacteria Bacillus subtilis and Bacillus circulans in formulated diets for rohu, Labeo rohita…
By A. Bairagi, K. Ghosh, S. Sen, A. Ray. 2004
190 citations recorded by the source corpus.
Nine isonitrogenous (35% crude protein approximately) and isocaloric (18.37 kJ g−1) experimental diets (RLL20–BCFL40) were formulated with either raw or treated (inoculated with fish intestinal bacteria) Leucaena leucocephala leaf meal at 20%, 30% and 40% levels replacing other ingredients partially from a fish meal based reference diet (RD). Two specific strains of fish intestinal bacteria, Bacillus subtilis (isolated from Cyprinus carpio) and B. circulans (isolated from Oreochromis mossambicus) having extracellular cellulolytic and amylolytic activities, were used to inoculate Leucaena leaf meal for 15 days at 37°C. The crude fibre, cellulose and hemicellulose contents and the antinutritional factors, tannin, phytic acid and mimosine in the leaf meal decreased due to inoculation. However, free amino acids and fatty acids increased in the treated leaf meal. The response of rohu, Labeo rohita, fingerlings fed the experimental diets for 80 days was compared with fish fed a RD. Both the inclusion level and type of Leucaena leaf meal in diets significantly affected the growth performance of rohu. Fish fed diets containing inoculated Leucaena leaf meal performed better in comparison with those with the RD. On the basis of growth response, feed conversion ratio, protein efficiency ratio and apparent net protein utilization, diet formulated with 30%Leucaena leaf meal inoculated with B. circulans resulted in the best performance of rohu fingerlings followed by diet with 40%B. subtilis inoculated Leucaena leaf meal. The apparent protein digestibility (APD) was better in fish fed diets containing B. circulans inoculated leaf meal. An increasing level of raw Leucaena leaf meal was associated with a decrease in the carcass protein content of rohu fingerlings. The activity of α-amylase increased with the increasing level of treated leaf meal in diets. Cellulase activity increased with increasing level of inclusion of raw leaf meal, and was comparatively lower in fish fed diets with treated leaf meal. Activities of protease and lipase were higher in fish fed the RD. The results showed that it is possible to incorporate Leucaena leaf meal inoculated with enzyme-producing fish intestinal bacteria in carp diets up to 40% level of inclusion.
Subfields: Aquatic Science, Immunology
View Paper: https://www.semanticscholar.org/paper/8d2cc782c5e588d511915c1a3a3c8a7804938144
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Aquatic Science
In Immunology
Citations in the corpus
- Probiotics in aquaculture: The need, principles and mechanisms of action and screening processes (2008, methodology): “The research approach consisted of either selecting and testing of LAB from the GIT of aquatic animals (Stoffels et al., 1992; Gildberg et al., 1995, 1997; Gildberg and Mikkelsen, 1998; Shiri Harzevili et al., 1998; Tovar et al., 2002; Bairagi et al., 2004), or using probiotics developed for terrest”
- Effect of synbiotics between Bacillus licheniformis and yeast extract on growth, hematological and biochemical indices of the Nile tilapia (Oreochromis niloticus) (2014, background): “licheniformis supplementation in the present study and previous studies (Bairagi et al., 2004; Bagheri et al., 2008).”
- Impact of rising feed ingredient prices on aquafeeds and aquaculture production (2009, background): “Biological enhancement through micro-organisms such as yeast and bacterial and fungal fermentations have been investigated to determine their capacity to reduce the effects of anti-nutrients in plant materials and current results demonstrate great potential of this method for removing anti-nutrients”
- The effect of supplementation with polysaccharides, nucleotides, acidifiers and Bacillus strains in fish meal and soy bean based diets on growth performance in juvenile turbot (Scophthalmus maximus) (2015, background): “In somefish species the dietary inclusion of Bacillus subtilis and B. licheniformis strains had a positive effect on growth performance (Bairagi et al., 2004; He et al., 2011; Kumar et al., 2006; Raida et al., 2003).”
- Microbial floc meal as a replacement ingredient for fish meal and soybean protein in shrimp feed (2009, background): “It is also speculated that microbial flocs are probiotics (Bairagi et al., 2002; Bairagi et al., 2004; Kesarcodi-Watson et al., 2008).”