The effect of pore size on cell adhesion in collagen-GAG scaffolds.
By F. O'Brien, B. Harley, I. Yannas, L. Gibson. Biomaterials, 2005
1,289 citations recorded by the source corpus.
The biological activity of scaffolds used in tissue engineering applications hypothetically depends on the density of available ligands, scaffold sites at which specific cell binding occurs. Ligand density is characterized by the composition of the scaffold, which defines the surface density of ligands, and by the specific surface area of the scaffold, which defines the total surface of the structure exposed to the cells. It has been previously shown that collagen-glycosaminoglycan (CG) scaffolds used for studies of skin regeneration were inactive when the mean pore size was either lower than 20 microm or higher than 120 microm (Proc. Natl. Acad. Sci., USA 86(3) (1989) 933). To study the relationship between cell attachment and viability in scaffolds and the scaffold structure, CG scaffolds with a constant composition and solid volume fraction (0.005), but with four different pore sizes corresponding to four levels of specific surface area were manufactured using a lyophilization technique. MC3T3-E1 mouse clonal osteogenic cells were seeded onto the four scaffold types and maintained in culture. At the experimental end point (24 or 48 h), the remaining viable cells were counted to determine the percent cell attachment. A significant difference in viable cell attachment was observed in scaffolds with different mean pore sizes after 24 and 48 h; however, there was no significant change in cell attachment between 24 and 48 h for any group. The fraction of viable cells attached to the CG scaffold decreased with increasing mean pore size, increasing linearly (R2 = 0.95, 0.91 at 24 and 48 h, respectively) with the specific surface area of the scaffold. The strong correlation between the scaffold specific surface area and cell attachment indicates that cell attachment and viability are primarily influenced by scaffold specific surface area over this range (95.9-150.5 microm) of pore sizes for MC3T3 cells.
Subfields: Cell Biology, Rehabilitation, Urology
View Paper: https://www.semanticscholar.org/paper/8bcc466262959ff934e80d833004545aa9844cb2
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Citations in the corpus
- Intrinsic extracellular matrix properties regulate stem cell differentiation. (2010, background): “, 2005; Ruiz and Chen, 2008) as well as micropores of varying size within a scaffold (O’Brien et al., 2005) appear to directly regulate force production in stem cells, indicating that there may exist a gradual transition from a highly tensed, spread cell to a low tension, more rounded cell that cont”
- Porous scaffolds for bone regeneration (2020, background): “pore size of collageneGAG scaffolds in vitro [52].”
- State of the art and future directions of scaffold‐based bone engineering from a biomaterials perspective (2007, background): “A review of the current bone engineering literature suggests that the scaffolds should fulfil a range of minimal requirements (Zeltinger et al., 2001; Karande et al., 2004; Karageorgiou et al., 2005; O’Brien et al., 2005).”
- Understanding the effect of mean pore size on cell activity in collagen-glycosaminoglycan scaffolds (2010, methodology): “This study showed that by varying the temperature of freezing from -40 to -10°C it was possible to produce homogenous CG scaffolds with mean pore sizes ranging from 96–151 μm.(6) A cellular solid is one made up of an interconnecting porous network and cellular solids modeling techniques can be used ”
- The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for bone tissue engineering. (2010, methodology): “to be optimal [1,12,14,15,27], however, the peak disappears at the later time point of 7 days due to reduced cell proliferation and poor migration.”