Preventing re-replication of chromosomal DNA

By J. Blow, Anindya Dutta. Nature reviews. Molecular cell biology, 2005

715 citations recorded by the source corpus.

To ensure its duplication, chromosomal DNA must be precisely duplicated in each cell cycle, with no sections left unreplicated, and no sections replicated more than once. Eukaryotic cells achieve this by dividing replication into two non-overlapping phases. During late mitosis and G1, replication origins are 'licensed' for replication by loading the minichromosome maintenance (Mcm) 2–7 proteins to form a pre-replicative complex. Mcm2–7 proteins are then essential for initiating and elongating replication forks during S phase. Recent data have provided biochemical and structural insight into the process of replication licensing and the mechanisms that regulate it during the cell cycle.

Subfields: Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/8b54026b4d6b54842f605736ed7f54b5d5ebc1db

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In Cell Biology

1. Debashish Chowdhury
2. Johannes C. Walter
3. G. Williams
4. David Glover
5. J. Diffley
6. K. Stoeber
7. A. Kriegstein

In Molecular Biology

1. R. Margueron
2. D. Reinberg
3. J. Harper
4. Debashish Chowdhury
5. Johannes C. Walter
6. G. Williams
7. J. Diffley
8. A. Hanyu

Citations in the corpus

• Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. (2008, background): “The entire or numerous partial cDNA species of human Cdt1 (GenBank: NM_030928) or human Geminin (GenBank: NM_015895) were amplified using primers containing 50-XhoI and 30-XbaI sites, and digested products were cloned in-frame into the XhoI/XbaI sites of pcDNA3/mKO2 or pcDNA3/mAG.”
• Centrosome biogenesis and function: centrosomics brings new understanding (2007, background): “However, whereas the molecular mechanisms that restrict DNA replication to a single round (known as DNA licensing ) are well understood (reviewed in Ref”
• Stochastic mechano-chemical kinetics of molecular motors: A multidisciplinary enterprise from a physicist’s perspective (2012, background): “In other words, the replication origins get “licence” to “fire” once, and only once, in a cell cycle [1640, 1641, 1642, 1643].”
• The cell cycle and cancer (2012, background): “The key event in suppressing relicensing of origins is the inactivation of the MCM loading factor Cdt1 through two mechanisms [57].”
• A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1. (2006, background): “Effects of HsCdt2 Depletion on the Cell Cycle Inhibition of Cdt1 activity during S phase by Geminin and proteolysis is essential to maintain once-per-cell-cycle regulation of DNA replication in metazoans (Blow and Dutta, 2005).”
• Chromatin Structure and the Inheritance of Epigenetic Information (2010, background): “The activation step leads to the production of a large complex (the replisome protein complex) that contains multiple proteins required for the formation of MCM helicase activity, which catalyses DNA unwindin”
• Concerted Loading of Mcm2-7 Double Hexamers Around DNA during DNA Replication Origin Licensing (2009, background): “A two-step mechanism underpins once-per-cell-cycle replication in eukaryotes (Bell and Dutta, 2002; Blow and Dutta, 2005; Diffley, 2004).”