Super-Resolution Video Microscopy of Live Cells by Structured Illumination
By P. Kner, Bryant B. Chhun, E. Griffis, Lukman Winoto, Mats Gustafsson. Nature Methods, 2009
724 citations recorded by the source corpus.
Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.
Subfields: Biomedical Engineering, Biophysics, Structural Biology
View Paper: https://www.semanticscholar.org/paper/8b2e64d9f877d6069248f6725a8819425c775923
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In Biomedical Engineering
- Michael A. D. Taylor
- Warwick P. Bowen
- Lothar Schermelleh
- Rainer Heintzmann
- H. Leonhardt
- Michael W. Davidson
- M. Gerl
- K. Simons
In Biophysics
- Michael A. D. Taylor
- Warwick P. Bowen
- Eric Betzig
- Lothar Schermelleh
- Rainer Heintzmann
- H. Leonhardt
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In Structural Biology
- Warwick P. Bowen
- Michael A. D. Taylor
- Lothar Schermelleh
- Rainer Heintzmann
- H. Leonhardt
- Stefan W. Hell
Citations in the corpus
- Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI) (2009, methodology): “Recently, superresolution microscopy at 11 Hz has been demonstrated by using SIM, achieving a 2-fold increased lateral resolution (14).”
- Quantum metrology and its application in biology (2014, methodology): “Furthermore, these approaches are based on a scanning point focus, and therefore lack the speed of parallel imaging [184].”
- Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics (2015, background): “The beam is then expanded to a 1/e(2) diameter of 12 mm and sent to a phase-only modulator (13) consisting of a polarizing beam splitter, a achromatic half-wave plate (HWP; Bolder Vision Optik, BVO AHWP3), and a ferroelectric spatial light modulator (SLM; ForthDimensionDisplays, SXGA3DM).”
- A guide to super-resolution fluorescence microscopy (2010, background): “Recently, the first steps toward the imaging of living cells with SIM were taken to visualize mitochondria, tubulin, and kinesin dynamics in living cells (Hirvonen et al., 2009; Kner et al., 2009).”