A method to separate plant roots from soil and analyze root surface area

By J. Benjamin, D. Nielsen. Plant and Soil, 2004

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Fine-root morphological and growth traits in a Turkey-oak stand in relation to seasonal changes in soil moisture in the Southern Apennines, Italy (2012, methodology): “For processing, each sample was placed in a nylon bag (300-lm mesh) that was contained in a plastic cylinder (6-mm mesh) and washed automatically using a washing machine (adapted by Benjamin and Nielsen 2004).”
Water deficit effects on root distribution of soybean, field pea and chickpea (2006, methodology): “Roots were washed from the soil cores and measurements were made using digital image analysis techniques as described in Benjamin and Nielsen (2004).”
Plant phenotyping: from bean weighing to image analysis (2015, methodology): “In other approaches, soil cores are taken in order to sample vertical root length densities or weights, sometimes using semiautomatic extraction methods [112,113].”
Root Phenotyping for Drought Tolerance: A Review (2018, methodology): “Among the different phenotyping techniques being used in the field, the soil core method and standard excavation method are considered as the best techniques to explore density, depth and angle of root [112,114].”