Nonlinear magic: multiphoton microscopy in the biosciences

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Spectral imaging: Principles and applications (2006, background): “(21) has extended the excitation method to multiphoton excitation (22) and has shown how it can be used to eliminate autofluorescence and distinguish dyes with similar emission spectra.”
Interpreting second-harmonic generation images of collagen I fibrils. (2005, methodology): “However, with a beam that is stationary (for seconds to minutes) for spectroscopic measurements (Zipfel et al., 2003a) or SHG diffraction images, an artifactual photoinduced green fluorescence was sometimes observed to appear, especially with illumination wavelengths ,750 nm.”
Going deeper than microscopy: the optical imaging frontier in biology (2010, methodology): “The most widely used technologies for in vivo microscopy of tissues are confocal and two- or multiphoton (2P/MP) microscop”
Determination of supramolecular structure and spatial distribution of protein complexes in living cells (2009, methodology): “Its excitation scheme is similar to most two-photon microscope”