Producing primate embryonic stem cells by somatic cell nuclear transfer

By J. Byrne, D. Pedersen, L. Clepper, M. Nelson, W. Sanger, S. Gokhale, Don P. Wolf, S. Mitalipov. Nature, 2007

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Senescence impairs successful reprogramming to pluripotent stem cells. (2009, background): “Besides, although Byrne and colleagues have successfully derived nuclear-transfer ES cells from primates by improving the SCNT protocol (Byrne et al., 2007), the same was never achieved with human cells.”
Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency. (2008, positive): “The success of somatic cell nuclear transfer (SCNT) experiments in mammalian species provided proof that the epigenetic state of adult differentiated cells is not fixed but remains pliable for reprogramming by factors present in the oocyte (Byrne et al., 2007; Jaenisch and Young, 2008; Wakayama and ”
Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer (2013, methodology): “2% [60 out of 63]) survived MII spindle removal conducted under polarized microscopy (Oosight) (Byrne et al., 2007; Sparman et al., 2009), and nuclear donor fibroblasts were introduced with 100% efficacy using HVJ-E based fusion.”
Generation of human induced pluripotent stem cells from dermal fibroblasts (2008, background): “Recent work showing that primate ES cells can be derived by somatic cell nuclear transfer (SCNT) from somatic cells opens the door to the possibility that SCNT of human cells will soon allow for the generation of ‘‘patient-specific’’ ES cells (1).”