RNA Stable Isotope Probing, a Novel Means of Linking Microbial Community Function to Phylogeny

By M. Manefield, Andrew S. Whiteley, R. Griffiths, M. Bailey. Applied and Environmental Microbiology, 2002

636 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/80210fc42ddde1fcd924270e896b1fe86ce525c7

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Citations in the corpus

Exploring methanotroph diversity in acidic northern wetlands: Molecular and cultivation-based studies (2009, background): “Both DNA and RNA were extracted from the peat samples, pmoA genes were amplified from DNA and cDNA, which was reverse-transcribed from mRNA, and the diversity of pmoA genes and gene transcripts was determined using a pmoA diagnostic microarray.”
Darkness visible: reflections on underground ecology (2005, neutral): “New technologies will be the key to progress: one novel approach that has enormous potential is stable isotope probing (SIP; Radajewski et al. 2000; Manefield et al. 2002), in which a resource labelled with the stable isotope (13)C is introduced to a system, and nucleic acids are subsequently extrac”
The fat that matters: Soil food web analysis using fatty acids and their carbon stable isotope signature (2010, methodology): “To date studies using NA-SIP have investigated microorganisms actively involved in specific metabolic processes (Radajweski et al., 2000; Manefield et al., 2002) or microbial food webs (Lueders et al., 2004, 2006) and as yet there has been no application to higher trophic levels.”
Identification of in situ 2,4-dichlorophenoxyacetic acid-degrading soil microorganisms using DNA-stable isotope probing (2007, neutral): “, 2003; Yu and Chu, 2005), phenol (DeRito et al., 2005; Manefield et al., 2002; Padmanabhan et al., 2003), methanol (Lueders et al.”
Biodiversity and ecosystem function in soil (2005, background): “Several groups within the SBP are applying stable-isotope probing and are developing it to use mRNA rather than DNA (Manefield et al. 2002), as RNA is more labile and so likely to reveal the taxa that are actively metabolizing C in situ.”
Stable isotope probing — linking microbial identity to function (2005, methodology): “RNA-based SIP The recovery and analysis of RNA moleculesafter SIP has recently been developed and has some advantages over DNA-SI”
Molecular approaches to study the insect gut symbiotic microbiota at the ‘omics’ age (2010, methodology): “In addition to gene-targeting PCR of DNA samples, reverse transcriptase PCR (RT-PCR) from RNA has also been employed to clone genes from environmental samples (Manefield et al., 2002).”
Soil Microbial Community Analysis Using Denaturing Gradient Gel Electrophoresis (2007, methodology): “Molecular techniques such as PCR-DGGE, however, are providing cost-effective means to examine and compare a large number of samples within short time frames.”