Genome engineering using CRISPR-Cas9 system.

By Le Cong, Feng Zhang. Methods in molecular biology, 2015

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GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases (2014, neutral): “nature biotechnology volume 33 NumBeR 2 FeBRuARY 2015 197 25. Ran, F.A. et al. Genome engineering using the CRISPR-Cas9 system. Nat. Protoc. 8, 2281–2308 (2013). 26. Heigwer, F., Kerr, G. & Boutros, M. E-CRISP: fast CRISPR target site identification. Nat. Methods 11, 122–123 (2014). 27. Fu, Y., Sand”
Repurposing the CRISPR-Cas9 system for targeted DNA methylation (2016, methodology): “The CRISPR-Cas9 system can target any 20 bp genomic DNA sequence which is followed by the PAM sequence 5′NGG-3′, which occurs on average every 8–12 bp in the human genome (2).”
The PINK1-PARKIN mitochondrial ubiquitylation pathway drives a program of TBK1 activation and recruitment of OPTN and NDP52 to promote mitophagy (2015, methodology): “, 2014) were gene edited (Ran et al., 2013) to ablate OPTN, NDP52, and TBK1 or PINK1 and, in some cases, reconstituted with the indicated proteins using a Flp-In construct in which PARKIN is expressed via an Internal Ribosomal Entry Site.”
Alternative Wnt Signaling Activates YAP/TAZ (2015, methodology): “Together, our work establishes YAP/TAZ as critical mediators of alternative”