Myosin II dynamics are regulated by tension in intercalating cells
By R. Fernandez-Gonzalez, Sérgio de Matos Simoes, Jens-Christian Röper, Suzanne Eaton, J. Zallen. Developmental Cell, 2009
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Summary Axis elongation in Drosophila occurs through polarized cell rearrangements driven by actomyosin contractility. Myosin II promotes neighbor exchange through the contraction of single cell boundaries, while the contraction of myosin II structures spanning multiple pairs of cells leads to rosette formation. Here we show that actomyosin cables form at a higher frequency than expected by chance during elongation, indicating that cable assembly is an active process. Multicellular cables are sites of increased mechanical tension as measured by laser ablation. Fluorescence recovery after photobleaching experiments show that myosin II is stabilized at the cortex in regions of increased tension. Myosin II is recruited in response to an ectopic force and relieving tension leads to a rapid loss of myosin, indicating that tension is necessary and sufficient for cortical myosin localization. These results demonstrate that myosin II dynamics are regulated by tension in a positive feedback loop that leads to actomyosin cable formation and efficient tissue elongation.
Subfields: Cell Biology, Cellular and Molecular Neuroscience, Ecology, Evolution, Behavior and Systematics
View Paper: https://www.semanticscholar.org/paper/7f3074ae6176f66d7002675a47bbc90bac94fdce
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Citations in the corpus
- Balancing forces: architectural control of mechanotransduction (2011, neutral): “Applying force to a cell within an embryo with a micropipette is sufficient to recruit myosin in 1–3 minutes to the apical cortex, whereas relieving tension with laser-ablation leads to a rapid loss of myosin from the corte”
- Adherens junctions: from molecules to morphogenesis (2010, background): “, but forces across AJ-mediated cell–cell contacts can also recruit additional myosi”
- Mechanical control of tissue and organ development (2010, background): “Myosin II is also enriched at sites of tension application at the surface of intercalating cells (Fernandez-Gonzalez et al., 2009).”
- Forces in tissue morphogenesis and patterning. (2013, positive): “In turn, MyoIImediatedcortical tension induces furtherMyoII recruitmentonAP junctions of adjacent cells, leading to the formation of supracellular MyoII cables that trigger simultaneous intercalations of multiple cells contributing togerm-bandelongation (Blankenshipet al., 2006; Fernandez-Gonzalez e”