Automated structure solution with autoSHARP.

By C. Vonrhein, E. Blanc, P. Roversi, Gerard Bricogne. 2007

1,002 citations recorded by the source corpus.

Subfields: Materials Chemistry, Mechanics of Materials

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In Materials Chemistry

Citations in the corpus

Features and development of Coot (2010, neutral): “A two-dimensional orientation search Map data labels output by common crystallography software, including DM (Zhang et al. , 1997), Parrot (Cowtan, 2010), Pirate (Cowtan, 2000) and autoSHARP (Vonrhein et al. , 2007). is performed to optimize the orientation of the cylinder.”
Overview of the CCP4 suite and current developments (2011, methodology): “While we focus here on the CCP4 software suite, we would emphasize that comparable functionality is available in other software packages such as SHARP/autoSHARP (Vonrhein et al., 2007), SHELX (Sheldrick, 2008), ARP/wARP (Langer et al., 2008), PHENIX (Adams et al., 2010) and many others.”
Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion (2016, methodology): “Initial phases were obtained using the SAD method in autoSHARP (51), and Buccaneer, as implemented in autoSHARP, was used for initial model building (52).”
Structural Insight Into Conformational Changes Induced by ATP Binding in a Type III Secretion-Associated ATPase From Shigella flexneri (2018, methodology): “The AUTOSHARP/SHARP program was used to locate the Se atoms and calculate the initial phase to produce an interpretable electron density map (Vonrhein et al., 2007).”
Structural insights into the assembly of the histone deacetylase-associated Sin3L/Rpd3L corepressor complex (2015, methodology): “Experimental phases were determinedwith AutoSHARP (55) by single isomorphous replacement with anomalous scattering from five Sds3 LnkCC290-174 SeMet derivative crystals, which were grown and frozen as the native crystals.”
Graphical tools for macromolecular crystallography in PHENIX (2012, background): “…or all of the computational ‘pipeline’ nearly unattended have been proposed, many focused around linking together existing components with command-line scripts or HTML interfaces (Brun-zelle et al. , 2003; Holton & Alber, 2004; Kroemer et al. , 2004; Panjikar et al. , 2005; Vonrhein et al. , 2007).”
The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism. (2013, methodology): “X-ray diffraction data were collected at the Australian Synchrotron beamline MX2 at 100K. Data were processed with HKL2000 (Otwinowski and Minor, 1997) and the structure solved by SAD with autoSHARP (Vonrhein et al., 2007) with data collected from crystals soaked in 2 mM UO2(NO3)2 before freezing.”
The Phenix Software for Automated Determination of Macromolecular Structures (2011, methodology): “Finally, many of the automated methods have been brought together in automated structure solution pipelines such as AutoRickshaw[12], HKL3000[13], Crank[14] and AutoSHARP[15] plus AutoBUSTER[16].”
Structural mechanism of cytosolic DNA sensing by cGAS (2013, positive): “AutoSHARP was used to locate Se sites (SAD dataset) and to produce an initial solvent flattened map 33 .”
Structural and Biophysical Studies of the Human IL-7/IL-7Rα Complex (2009, methodology): “0 Å (Bricogne et al., 2003; Vonrhein et al., 2007).”