Mouse and rat BDNF gene structure and expression revisited

By Tamara Aid, A. Kazantseva, M. Piirsoo, Kaia Palm, T. Timmusk. Journal of Neuroscience Research, 2006

995 citations recorded by the source corpus.

Brain‐derived neurotrophic factor (BDNF) has important functions in the development of the nervous system and in brain plasticity‐related processes such as memory, learning, and drug addiction. Despite the fact that the function and regulation of rodent BDNF gene expression have received close attention during the last decade, knowledge of the structural organization of mouse and rat BDNF gene has remained incomplete. We have identified and characterized several mouse and rat BDNF transcripts containing novel 5′ untranslated exons and introduced a new numbering system for mouse and rat BDNF exons. According to our results both mouse and rat BDNF gene consist of eight 5′ untranslated exons and one protein coding 3′ exon. Transcription of the gene results in BDNF transcripts containing one of the eight 5′ exons spliced to the protein coding exon and in a transcript containing only 5′ extended protein coding exon. We also report the distinct tissue‐specific expression profiles of each of the mouse and rat 5′ exon‐specific transcripts in different brain regions and nonneural tissues. In addition, we show that kainic acid‐induced seizures that lead to changes in cellular Ca2+ levels as well as inhibition of DNA methylation and histone deacetylation contribute to the differential regulation of the expression of BDNF transcripts. Finally, we confirm that mouse and rat BDNF gene loci do not encode antisense mRNA transcripts, suggesting that mechanisms of regulation for rodent and human BDNF genes differ substantially. © 2006 Wiley‐Liss, Inc.

Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience, Developmental Neuroscience

View Paper: https://www.semanticscholar.org/paper/7d76a93c05cd5ec66e7da11a931ce774c0df41a7

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Citations in the corpus

Molecular mechanisms and potential therapeutical targets in Huntington's disease. (2010, background): “The different promoter-like sequences upstream of exons I-IX act independently and modulate the transcription of mRNA I-IX in a stimulus- and developmental-specific manner (2, 453, 580, 581).”
EPIGENETIC REGULATION OF BDNF GENE TRANSCRIPTION IN THE CONSOLIDATION OF FEAR MEMORY (2008, background): “The bdnf gene consists of nine 5 noncoding exons each linked to individual promoter regions, and a 3 coding exon (IX), which codes for the BDNF preprotein amino acid sequence (Liu et al., 2006; Aid et al., 2007).”
BDNF — a key transducer of antidepressant effects (2015, background): “The BDNF gene has nine promoters that drive expression of distinct Bdnf transcripts that each encode the same BDNF protein (Aid et al., 2007; Pruunsild et al., 2007).”
Brain-Derived Neurotrophic Factor and Neuropsychiatric Disorders (2012, background): “The gene structure of BDNF is quite complex and has recently been reviewed for rodent (Liu et al., 2006; Aid et al., 2007) and human (Liu et al.”
New insights in the biology of BDNF synthesis and release: implications in CNS function (2009, positive): “…The Bdnf gene is comprised of at least eight distinct promoters that initiate transcription of multiple distinct mRNA transcripts, each of which contains an alternative 5 exon spliced to a common 3 coding exon that contains the entire open reading frame for the BDNF protein (Aid et al., 2007).”
From synapse to nucleus: calcium-dependent gene transcription in the control of synapse development and function. (2008, background): “The alternative promoters together with alternative splicing and polyadenylation result in the production of at least 18 distinct Bdnf mRNAs, each of which encodes an identical protein (Aid et al., 2007).”
BDNF-induced local protein synthesis and synaptic plasticity. (2014, positive): “As many as 11 different splicevariants of BDNF mRNA were described in both humans and rodents (Aid et al., 2007).”
Networks of anatomical covariance (2013, background): “…2012; Schmitt et al., 2008, 2009, 2010), normal development and aging (Chen et al., 2011a, 2011b; Raz et al., 2005), mutual trophic influences (Aid et al., 2007; Ferrer et al., 1995) or experience-related plasticity (Bermudez et al., 2009; Draganski et al., 2004; Driemeyer et al., 2008; Haier…”
Brain-derived neurotrophic factor and its clinical implications (2015, methodology): “Arrangement of introns and exons on BDNF genes is determined by analyzing genomic and mRNA sequence using bioinformatics, RACE, and RT-PCR [17] I III II IV V Poly(A) signals ATG”