Construction of implantable optical fibers for long-term optogenetic manipulation of neural circuits

By Dennis R. Sparta, A. Stamatakis, Jana L Phillips, N. Hovelsø, Ruud van Zessen, Garret D. Stuber. Nature Protocols, 2011

390 citations recorded by the source corpus.

In vivo optogenetic strategies have redefined our ability to assay how neural circuits govern behavior. Although acutely implanted optical fibers have previously been used in such studies, long-term control over neuronal activity has been largely unachievable. Here we describe a method to construct implantable optical fibers to readily manipulate neural circuit elements with minimal tissue damage or change in light output over time (weeks to months). Implanted optical fibers readily interface with in vivo electrophysiological arrays or electrochemical detection electrodes. The procedure described here, from implant construction to the start of behavioral experimentation, can be completed in approximately 2–6 weeks. Successful use of implantable optical fibers will allow for long-term control of mammalian neural circuits in vivo, which is integral to the study of the neurobiology of behavior.

Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience

View Paper: https://www.semanticscholar.org/paper/7be170a45786a09b5c83809380c0bb1d5feb721e

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Citations in the corpus

Flexible near field wireless optoelectronics as subdermal implants for broad applications in optogenetics (2017, positive): “This methodology is considered as essential for current efforts in neuroscience research largely due to its capabilities for sophisticated functional studies in the central and peripheral nervous systems (Bonin et al., 2015; Boyden et al., 2005; Iyer et al., 2014; Kim et al., 2013; Sparta et al., 20”
Differential control of learning and anxiety along the dorso-ventral axis of the dentate gyrus (2013, methodology): “Construction of Optical Fibers We employed use of published techniques for the construction of chronically dwelling optical fibers and patch cables for behavioral procedures (Sparta et al., 2012).”
Activation of VTA GABA neurons disrupts reward consumption (2012, positive): “Preparation of optical fibers For a detailed account of the construction of implantable optical fibers and optical patch cables, see (Sparta et al., 2011).”
Hippocampal Memory Traces Are Differentially Modulated by Experience, Time, and Adult Neurogenesis (2014, methodology): “Construction of Optical Fibers We employed use of published techniques for the construction of chronically dwelling optical fibers and patch cables for behavioral procedures (Sparta et al., 2012).”