In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes

By Wigard P. Kloosterman, Erno Wienholds, E. Bruijn, Sakari Kauppinen, Ronald H. A. Plasterk. Nature Methods, 2005

615 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7b7bc60f1a53fc65fa75b7fbe13b138bce09d7fc

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Citations in the corpus

Plant microRNAs: new players in functional genomics (2013, neutral): “…(ISH) has emerged as a powerful technique that combines molecular biological techniques with histological and cytological approaches for detection of specific nucleic acid sequences in samples at the cellular, tissue, and even whole organism levels (Valoczi et al. 2004; Kloosterman et al. 2006).”
miRNAs as Biomarkers in Disease: Latest Findings Regarding Their Role in Diagnosis and Prognosis (2020, background): “miRNAs can be detected using various specific and sensitive approaches, including Northern blot analysis [294,295], in situ hybridization [296], real-time PCR [95,297], miRNA microarray [298,299] and next-generation sequencing (NGS) [300], and some of them are already used as diagnostic or prognosti”
A mammalian microRNA expression atlas based on small RNA library sequencing. (2007, background): “The expression patterns of miRNAs were recently examined by in situ hybridization analysis during development of zebrafish and mouse, and in human brain samples (Wienholds et al., 2005; Kloosterman et al., 2006a, 2006b; Nelson et al., 2006).”
microRNA as Biomarkers and Diagnostics (2016, methodology): “…have been used to detect miRNAs, including Northern blot analysis (Sempere et al., 2004; Valoczi et al., 2004), in situ hybridization analysis (Kloosterman et al., 2006), real-time PCR analysis (Chen et al., 2005; Wang et al., 2009), miRNA microarray (Thomson et al., 2004; Wang et al.,…”
What determines growth potential and juvenile quality of farmed fish species (2013, background): “…or myosin light chains (Moutou et al. 2005; Silva et al. 2010) specific to myotubes and immature muscle fibres, which can be used as markers for hyperplastic growth. miRNAs can also be detected by in situ hybridization with locked nucleic acid (LNA)-modified DNA probes (Kloosterman et al. 2006).”
Extensive post-transcriptional regulation of microRNAs and its implications for cancer. (2006, background): “During mid- to late embryonic development, large numbers of miRNAs are induced in temporal and spatial patterns (Kloosterman et al. 2006).”
Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver (2007, positive): “(20) in which highly sensitive and specific detection of miR-206 and miR-124a in zebrafish embryos by whole-mount ISH could be achieved using shortened LNA versions (16- to 14-mers) complementary to the 50-end of the miRNA.”
MicroRNA detection by northern blotting using locked nucleic acid probes (2008, background): “As LNA-modified oligonucleotide probes can also be used for in situ hybridizatio”