Induced Pluripotent Stem Cells Generated from Patients with ALS Can Be Differentiated into Motor Neurons
By J. Dimos, Kit T. Rodolfa, Kathy K. Niakan, L. Weisenthal, H. Mitsumoto, Wendy K. Chung, Gist F. Croft, G. Saphier, Rudolph L. Leibel, R. Goland and 3 more. Science, 2008
1,994 citations recorded by the source corpus.
The generation of pluripotent stem cells from an individual patient would enable the large-scale production of the cell types affected by that patient's disease. These cells could in turn be used for disease modeling, drug discovery, and eventually autologous cell replacement therapies. Although recent studies have demonstrated the reprogramming of human fibroblasts to a pluripotent state, it remains unclear whether these induced pluripotent stem (iPS) cells can be produced directly from elderly patients with chronic disease. We have generated iPS cells from an 82-year-old woman diagnosed with a familial form of amyotrophic lateral sclerosis (ALS). These patient-specific iPS cells possess properties of embryonic stem cells and were successfully directed to differentiate into motor neurons, the cell type destroyed in ALS.
Subfields: Molecular Biology, Neurology, Physiology
View Paper: https://www.semanticscholar.org/paper/7a931c9c23ed2ea6c5ba27b8cca11910f3b8b252
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In Molecular Biology
- K. Hochedlinger
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- R. Jaenisch
- Kathrin Plath
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In Neurology
- Fred H. Gage
- T. Palmer
- P. Carpentier
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- Florent Ginhoux
- Hongjun Song
In Physiology
- R. Jaenisch
- K. Hochedlinger
- M. Stadtfeld
- Fred H. Gage
- S. Yamanaka
- Florent Ginhoux
- D. Hockemeyer
- Yangling Mu
Citations in the corpus
- Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations (2011, positive): “…of human somatic cells into an embryonic stem cell-like state followed by differentiation into any cell type of the body, is being developed as a key component of such in vitro disease modeling (Dimos et al., 2008; Park et al., 2008; Soldner et al., 2009; Takahashi et al., 2007; Yu et al., 2007).”
- Immune Influence on Adult Neural Stem Cell Regulation and Function (2009, positive): “iPSC lines have been created from patients with neurological diseases such as PD, amyotrophic lateral sclerosis (ALS), and spinal muscular atrophy (SMA) (Dimos et al., 2008; Ebert et al., 2009; Soldner et al., 2009).”
- A fresh look at iPS cells. (2009, background): “For example, motor neurons alone derived from ALS patient-specific iPS cells may not be able to reconstitute full disease pathogenesis as they may need to interact with glial cells (Di Giorgio et al., 2007).”
- Stem Cell Organoid Engineering (2016, positive): “…et al., 2013), midbrain dopaminergic neurons (Chambers et al., 2009; Lee et al., 2000; Perrier et al., 2004; Yan et al., 2005), and spinal cord motor neurons (Dimos et al., 2008; Li et al., 2005; Soundararajan et al., 2006; Wichterle et al., 2002) (see Petros et al., 2011 for review).”