Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin.

By A. Bolotin, Benoit Quinquis, A. Sorokin, S. Ehrlich. Microbiology, 2005

882 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/7929e552986deebd76373f38a508109d479bdbf3

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Citations in the corpus

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences (2014, unknown): “Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute a bacterial defense system that cleaves invading foreign nucleic acids (1–8).”
Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus (2007, background): “Sequence comparison between the three typical repeats showed that CRISPR1 and CRISPR2 share 30.6% identity, while CRISPR1 and CRISPR3 are 52.8% identical, and CRISPR2 and CRISPR3 share 47.2% nucleotides.”
Short motif sequences determine the targets of the prokaryotic CRISPR defence system. (2009, background): “Another homology-based analysis of proto-spacer regions (Bolotin et al., 2005) showed for the CRISPR-16 loci of S. thermophilus the consensus PuPyAAA, which is also shorter than that found by Deveau et al. (2008) in the same species (NNAGAAW).”
Delivering CRISPR: a review of the challenges and approaches (2018, background): “The second significant advance occurred in 2005 when Mojica et al. (2005), Pourcel et al. (2005), and Bolotin et al. (2005) all independently reported that the nonrepeating CRISPR spacers contained sequences derived from foreign chromosomal DNA, specifically DNA from bacteriophages.”
CRISPR/Cas system and its role in phage-bacteria interactions. (2010, background): “thermophilus strains match a sequence already available in databases (12), whereas in other microbial systems it is only 2% (65).”
CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats (2007, background): “The originality of spacers is that they apparently derive from conjugative plasmids or bacteriophages (2,9–11).”
CRISPR Gene Therapy: Applications, Limitations, and Implications for the Future (2020, background): “in the target DNA adjacent to the spacer, later known as the protospacer adjacent motif (PAM)—the sequence needed for Cas9 to recognize and bind its target DNA (25).”
The Biology of CRISPR-Cas: Backward and Forward. (2018, unknown): “A milestone in CRISPR research was the realization that the spacer sequences match plasmids and phage genomes, as it was the ﬁrst hint that CRISPR-Cas might function as a prokaryotic defense mechanism (Bolotin et al., 2005; Mojica et al., 2005; Pourcel et al., 2005).”
The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “, 1987), their biological function was not understood until 2005, when it was shown that the spacers were homologous to viral and plasmid sequences suggesting a role in adaptive immunity (Bolotin et al., 2005; Mojica et al., 2005; Pourcel et al., 2005).”
CRISPR/Cas9 in Genome Editing and Beyond. (2016, background): “In contrast to most known DNA-binding proteins, Cas9 is an RNA-guided nuclease whose sequence specificity largely arises from Watson–Crick base pairing between its guide RNA and the target DNA site, in addition to a direct interaction between Cas9 and a short protospaceradjacent motif (PAM) of DNA (”
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling (2014, background): “Recently, the RNA-guided endonuclease Cas9 from microbial type II CRISPR (clustered regularly interspaced short palindromic repeat) systems (previ- ously referred to as Cas5 or Csn1) (Barrangou et al., 2007; Bolotin et al., 2005; Deltcheva et al., 2011; Garneau et al., 2010; Gasiunas et al., 2012; J”