Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

By D. G. Gibson, G. A. Benders, C. Andrews-Pfannkoch, Evgeniya Denisova, Holly Baden-Tillson, J. Zaveri, Timothy B. Stockwell, Anushka Brownley, D. W. Thomas, Mikkel A. Algire and 7 more. Science, 2008

1,150 citations recorded by the source corpus.

Subfields: Ecology, Epidemiology, Microbiology

View Paper: https://www.semanticscholar.org/paper/77a66eb1852fdb842a84834f0cdc0e59cd1f3f2c

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. D. G. Gibson
2. S. Lemire
3. Hiroki Ando
4. Christopher A. Voigt
5. James J. Collins

Citations in the corpus

• A Synthetic Genetic Edge Detection Program (2009, background): “The construction of very large fragments of DNA (Cello et al., 2002; Chan et al., 2005; Endy, 2008; Gibson et al., 2008a; Gibson et al., 2008b; Smith et al., 2003; Tumpey et al., 2005) is no longer a limitation in the engineering of biological systems.”
• Design and synthesis of a minimal bacterial genome (2016, background): “three weeks, which is about two orders of magnitude faster than the first reported synthesis of a bacterial genome (by our group) in 2008 (7).”
• Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides (2009, background): “This study, together with our previous studies ( 17 ,18), demonstrates that entire genomes can be synthesized within a yeast cell beginning from a series of oligonucleotides that overlap by as few as 20 nucleotides.”
• DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways (2008, background): “In the final step, the complete synthetic genome was assembled by transformationassociated recombination cloning in yeast when the size of the synthetic molecule became too big to be stable in E. coli ( 29 ).”
• Automated Design of Synthetic Ribosome Binding Sites to Precisely Control Protein Expression (2009, neutral): “As it becomes possible to construct larger pieces of synthetic DNA 14, including whole genomes 15, automated methods for genetic circuit assembly and metabolic pathway optimization will be critically important.”
• A synchronized quorum of genetic clocks (2010, neutral): “On a larger scale, progress has been made towards the creation of entire genomes, providing new insights into what constitutes the minimal set of genes required for microbial life 19 .”