DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways
By Zengyi Shao, Hua Zhao, Huimin Zhao. Nucleic Acids Research, 2008
657 citations recorded by the source corpus.
The assembly of large recombinant DNA encoding a whole biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cerevisiae. We show that DNA assembler can rapidly assemble a functional d-xylose utilization pathway (∼9 kb DNA consisting of three genes), a functional zeaxanthin biosynthesis pathway (∼11 kb DNA consisting of five genes) and a functional combined d-xylose utilization and zeaxanthin biosynthesis pathway (∼19 kb consisting of eight genes) with high efficiencies (70–100%) either on a plasmid or on a yeast chromosome. As this new method only requires simple DNA preparation and one-step yeast transformation, it represents a powerful tool in the construction of biochemical pathways for synthetic biology, metabolic engineering and functional genomics studies.
Subfields: Biotechnology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/e8b5fad228a630811e837fd40ef251a31bcb536a
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Biotechnology
In Molecular Biology
Citations in the corpus
- Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides (2009, background): “Later work showed that six smaller fragments were also acceptable (19).”