A Protein Complex Network of Drosophila melanogaster

By K. G. Guruharsha, Jean‐François Rual, B. Zhai, J. Mintseris, P. Vaidya, Namita Vaidya, Chapman N Beekman, Christina Y Wong, David Y Rhee, Odise Cenaj and 14 more. Cell, 2011

652 citations recorded by the source corpus.

SUMMARY Determining the composition of protein complexes is an essential step towards understanding the cell as an integrated system. Using co-affinity purification coupled to mass spectrometry analysis, we examined protein associations involving nearly five thousand individual, FLAG-HA epitope-tagged Drosophila proteins. Stringent analysis of these data, based on a novel statistical framework to define individual protein-protein interactions, led to the generation of a Drosophila Protein interaction Map (DPiM) encompassing 556 protein complexes. The high quality of DPiM and its usefulness as a paradigm for metazoan proteomes is apparent from the recovery of many known complexes, significant enrichment for shared functional attributes and validation in human cells. DPiM defines potential novel members for several important protein complexes and assigns functional links to 586 protein-coding genes lacking previous experimental annotation. DPiM represents, to our knowledge, the largest metazoan protein complex map and provides a valuable resource for analysis of protein complex evolution.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/74f07f66072800434d02c2a6190a16ad2381106f

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

In Spectroscopy

Citations in the corpus

Extensive cross-regulation of post-transcriptional regulatory networks in Drosophila (2015, positive): “in the same cell line (Guruharsha et al. 2011).”
A Census of Human Soluble Protein Complexes (2012, neutral): “(C) Human complexes conserved in fly (Guruharsha et al., 2011) and yeast (Babu et al., 2012) (see Table S3 and Extended Experimental Procedures).”
FlyBase: a guided tour of highlighted features (2022, methodology): “2012), protein–protein interactomes (Guruharsha et al. 2011; Rhee et al. 2014; Shokri et al. 2019), and DNA-binding site data from ChIP-chip/ChIP-seq datasets from modENCODE and the Furlong lab (Negre et al.”
Multidimensional proteomics for cell biology (2015, neutral): “Such approaches, in conjunction with high-throughput cDNA cloning strategies, have been used to examine protein complexes on a proteome-wide scale 63–67 .”
The BioPlex Network: A Systematic Exploration of the Human Interactome (2015, neutral): “, 2006) and Drosophila (Guruharsha et al., 2011) interactions.”
Dual Proteome-scale Networks Reveal Cell-specific Remodeling of the Human Interactome (2020, background): “Though no single methodology can overcome all limitations, AP-MS excels for profiling interactomes – including yeast (Gavin et al., 2002; Ho et al., 2002; Krogan et al., 2006), Drosophila (Guruharsha et al., 2011), and human (Hein et al., 2015) – due to its sensitivity and its ability to detect inte”