A Census of Human Soluble Protein Complexes

By P. Havugimana, G. T. Hart, Tamás Nepusz, Haixuan Yang, Andrei L. Turinsky, Zhihua Li, Peggy I. Wang, D. Boutz, Vincent Fong, S. Phanse and 14 more. Cell, 2012

844 citations recorded by the source corpus.

SUMMARY Cellular processes often depend on stable physical associations between proteins. Despite recent progress, knowledge of the composition of human protein complexes remains limited. To close this gap, we applied an integrative global proteomic profiling approach, based on chromatographic separation of cultured human cell extracts into more than one thousand biochemical fractions which were subsequently analyzed by quantitative tandem mass spectrometry, to systematically identify a network of 13,993 high-confidence physical interactions among 3,006 stably-associated soluble human proteins. Most of the 622 putative protein complexes we report are linked to core biological processes, and encompass both candidate disease genes and unnanotated proteins to inform on mechanism. Strikingly, whereas larger multi-protein assemblies tend to be more extensively annotated and evolutionarily conserved, human protein complexes with 5 or fewer subunits are far more likely to be functionally un-annotated or restricted to vertebrates, suggesting more recent functional innovations.

Subfields: Cell Biology, Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/524253b5cc391a9f8e6a2244f73823662e46729d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cell Biology

In Molecular Biology

In Spectroscopy

Citations in the corpus

Multidimensional proteomics for cell biology (2015, methodology): “A recent study also showed that combining multiple chromato graphic separations could increase the resolution between different protein complexes 70 .”
OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization (2022, neutral): “The diversity of large-scale cell biology approaches is a solu-tion to this problem ( 6 , 8 , 9 , 11 , 31 , 70 , 77 – 80 ).”
A proteome-scale map of the human interactome network. (2014, background): “, 2012) and a co-fractionation map of ~14,000 potentially binary interactions (Co-Frac) (Havugimana et al., 2012).”
Principles of assembly reveal a periodic table of protein complexes (2015, background): “This knowledge can inform the interpretation of highthroughput interaction experiments (24) or”
The BioPlex Network: A Systematic Exploration of the Human Interactome (2015, positive): “Literature datasets included BioGRID, CORUM, GeneMania, STRING, and MINT, as well as interactions recently reported via yeast-two-hybrid (Rolland et al., 2014) and LC-MS correlation proﬁling (Havugimana et al., 2012).”