Universal primer set for the full-length amplification of all influenza A viruses
By E. Hoffmann, J. Stech, Yi Guan, R. Webster, D. Pérez. Archives of Virology, 2001
2,117 citations recorded by the source corpus.
Subfields: Epidemiology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/71cd76a801da510b3714043629e3bc5e13a3e1ce
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In Epidemiology
In Molecular Biology
- Frederic D. Bushman
- Hyun-Mi Pyo
- Qiang Liu
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- Hong-Su Park
- Alex J. Bowers
Citations in the corpus
- Single-Reaction Genomic Amplification Accelerates Sequencing and Vaccine Production for Classical and Swine Origin Human Influenza A Viruses (2009, methodology): “One primer set amplifies all vRNAs in a single reaction, whereas the best method to date requires eight sets of primers, which are used in separate RTPCRs (8).”
- Sera from Individuals with Narrowly Focused Influenza Virus Antibodies Rapidly Select Viral Escape Mutations In Ovo (2018, methodology): “Reverse transcription was performed using Superscript III First-Strand Reaction Mix (Thermo Fisher) and an equimolar mix of the 5=-HoffmannU12-A4 and 5=-Hoffmann-U12-G4 primers, which bind to the conserved U12 region present on each influenza virus gene segment (27).”
- Influenza A Virus Panhandle Structure Is Directly Involved in RIG-I Activation and Interferon Induction (2015, background): “The dsRNA formed by the partial complementarity of these conserved nucleotides was further extended by a fully complementary region (2 to 3 bp) whose sequence is segment specific among all IAV subtypes (45).”
- Spatial, Temporal, and Species Variation in Prevalence of Influenza A Viruses in Wild Migratory Birds (2007, methodology): “RT-PCR was performed using primers specific for the conserved non-coding regions of NA, essentially as described by others [38].”
- Characterization of a Novel Influenza A Virus Hemagglutinin Subtype (H16) Obtained from Black-Headed Gulls (2005, background): “RT-PCR specific for the conserved noncoding regions of avian influenza viruses was described by others ( 16 ).”