Comprehensive transposon mutant library of Pseudomonas aeruginosa

By Michael A. Jacobs, Ashley Alwood, I. Thaipisuttikul, D. Spencer, E. Haugen, Stephen Ernst, O. Will, R. Kaul, C. Raymond, R. Levy and 5 more. Proceedings of the National Academy of Sciences of the United States of America, 2003

1,224 citations recorded by the source corpus.

Subfields: Ecology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/712c3f2cc35276770226ccdb60499478ae5b91fd

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Citations in the corpus

Genome-scale models of bacterial metabolism: reconstruction and applications (2008, background): “In parallel to this, collections of knockout mutants are being built for a growing number of bacteria (Akerley et al ., 2002; Jacobs et al ., 2003; Kobayashi et al ., 2003; Baba et al ., 2006; Liberati et al ., 2006; Suzuki et al ., 2006; de Berardinis et al ., 2008).”
Tn-seq; high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms (2009, background): “Other large categories include uncharacterized genes (13) and transcriptional regulators (6).”
High-Resolution Phenotypic Profiling Defines Genes Essential for Mycobacterial Growth and Cholesterol Catabolism (2011, background): “[5,6] or conventional sequencing [7,8,9] to map the sites of transposon insertions in large libraries of random mutants and identify regions of the chromosome that were unable to sustain mutation.”
Pseudomonas genomes: diverse and adaptable. (2011, background): “Both Jacobs et al. (2003) and Lewenza et al. (2005) created saturated transposon mutant libraries in PAO1.”
DEG 5.0, a database of essential genes in both prokaryotes and eukaryotes (2008, background): “…subtilis (24), those determined by global transposon mutagenesis in Francisella novicida (25), Helicobacter pylori (26), Mycobacterium tuberculosis (27), Mycoplasma pulmonis (28) and Pseudomonas aeruginosa (29,30), and those determined by trapping lethal insertions in Salmonella typhimurium (31).”
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection (2006, background): “Comprehensive transposon mutagenesis of Pseudomonas aeruginosa was carried out by generating a large set (30100) of sequence-defined mutants (Jacobs et al, 2003).”
Involvement of Nitric Oxide in Biofilm Dispersal of Pseudomonas aeruginosa (2006, neutral): “numbers 6761 or 11788 and 4583 or 13703, respectively) (31).”
Identifying genetic determinants needed to establish a human gut symbiont in its habitat (2009, neutral): “This is consistent with genome-wide mutagenesis studies of Escherichia coli and Pseudomonas aeruginosa (Baba et al., 2006; Jacobs et al., 2003).”
A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. (2006, methodology): “2 kb tetracycline resistance-encoding transposon insertion from a strain of the comprehensive PAO1 transposon library (Jacobs et al., 2003) into our PAO1 laboratory strain.”
A Type VI Secretion System of Pseudomonas aeruginosa Targets a Toxin to Bacteria (2010, background): “Tsi2 Is an Essential Protein that Protects P. aeruginosa from Tse2 The lack of transposon insertions within the tse2/tsi2 locus in a published transposon insertion library of P. aeruginosa PAO1 suggested that these ORFs may be essential for viability of the organism (Jacobs et al., 2003).”
Identification of FleQ from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor (2008, background): “PAO1 Wild-type strain; twitching motility+ Jacobs et al. (2003) PAO1100 PAO1 derivative, in-frame deletion of wspF Hickman et al. (2005) fleQ::Tn5 PAO1 derivative, fleQ::Tn5; TcR. Jacobs et al. (2003) fleN::Tn5 PAO1 derivative, fleN::Tn5; TcR. Jacobs et al. (2003) PAO1110 fleQ::Tn5 with in-frame del”