A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis.

By Yanhai Yin, Dionne Vafeados, Yi Tao, S. Yoshida, T. Asami, Joanne Chory. Cell, 2005

771 citations recorded by the source corpus.

Brassinosteroids (BRs) signal through a plasma membrane-localized receptor kinase to regulate plant growth and development. We showed previously that a novel protein, BES1, accumulates in the nucleus in response to BRs, where it plays a role in BR-regulated gene expression; however, the mechanism by which BES1 regulates gene expression is unknown. In this study, we dissect BES1 subdomains and establish that BES1 is a transcription factor that binds to and activates BR target gene promoters both in vitro and in vivo. BES1 interacts with a basic helix-loop-helix protein, BIM1, to synergistically bind to E box (CANNTG) sequences present in many BR-induced promoters. Loss-of-function and gain-of-function mutants of BIM1 and its close family members display BR response phenotypes. Thus, BES1 defines a new class of plant-specific transcription factors that cooperate with transcription factors such as BIM1 to regulate BR-induced genes.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/c8b93fe95ec7f9e577be09f84d2319bca76c9825

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Tae-Wuk Kim
2. Yanhai Yin
3. Zhi-Yong Wang
4. Seung Yon Rhee
5. Hongkai Ji

In Plant Science

1. Tae-Wuk Kim
2. Yanhai Yin
3. Zhi-Yong Wang

Citations in the corpus

• Brassinosteroids: Multidimensional Regulators of Plant Growth, Development, and Stress Responses[OPEN] (2019, background): “active in the nucleus to control BR-responsive gene expression (He et al., 2002; Wang et al., 201 2002; Yin et al., 2002; Zhao et al., 2002; Yin et al., 2005; Yu et al., 2011).”
• Brassinosteroid signal transduction from receptor kinases to transcription factors. (2010, background): “While both BIM1 overexpression and knockdown experiments support a role in plant growth regulation, the phenotypes of these plants are subtle (96).”
• Growth-defense tradeoffs in plants: a balancing act to optimize fitness. (2014, neutral): “In the absence of BR, the glycogen-synthase-kinase-3-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates two nuclear-localized transcription factors, BRI1-EMS-SUPPRESSOR 1 (BES1) and BRASSINAZOLE-RESISTANT 1 (BZR1), to block activation of BR-responsive genes (He et al., 2002; Wang et al.”
• Integration of Brassinosteroid Signal Transduction with the Transcription Network for Plant Growth Regulation in Arabidopsis (2010, positive): “…EXORDIUM (EXO) that promotes cell expansion (Schroder et al., 2009), the HERK1 receptor kinase (Guo et al., 2009) and bHLH transcription factor BEE1 (Friedrichsen et al., 2002), which promote cell elongation, and IBH1 (Zhang et al., 2009) and AIF1 (Wang et al., 2009), which inhibit cell elongation.”