Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes.

By Marijn G. J. Ford, B. Pearse, M. Higgins, Y. Vallis, D. Owen, A. Gibson, C. Hopkins, P. Evans, H. McMahon. Science, 2001

782 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/6b19bb558418d825d782bd90a270e8fbf1cea25f

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Citations in the corpus

PIP(2) and proteins: interactions, organization, and information flow. (2002, background): “The PIP2-binding site in CALM (X-ray structure) is unusual in that it consists of an exposed cluster of lysines that do not form a binding pocket and, curiously, appear to be different from the PIP2-binding site in epsin (NMR structure).”
A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis (2011, background): “Most similar to clathrin were the PI(4,5)P2-binding epsin N-terminal homology domain (ENTH)/AP180 N-terminal homology domain (ANTH) adaptor proteins epsin and CALM [8,39], both of which directly bind clathrin.”
Regulated portals of entry into the cell (2003, background): “Insight into their specific functions in CCV formation has been gained through in vivo analyses after interfering with protein interactions by overexpression of truncated mutants or peptide microinjection, and more recently through in vitro analysis of their behaviour on model liposome”
Can I solve my structure by SAD phasing? Planning an experiment, scaling data and evaluating the useful anomalous correlation and anomalous signal (2016, neutral): “These were 1hf8 (Ford et al., 2001), 2ahy (Shi et al., 2006), 2b78, 2fdn (Dauter et al., 1997), 2hba (Cho et al., 2014), 2o0h (Sun et al., 2007), 2prx, 2wxw (Zhou et al., 2010), 3fki (Meyer et al., 2009), 3i5d (Kawate et al., 2009), 3iko (Nagy et al., 2009), 3k9g (Abendroth et al., 2011), 3km3…”
Clathrin-mediated endocytosis in AP-2–depleted cells (2003, background): “…Hip1, Dab2, and ARH have all been shown to bind not only to AP-2, but also to PIP 2 and to clathrin, indicating that they may be able to bind to the plasma membrane and to recruit clathrin in an AP-2–independent manner (Gaidarov et al., 1999; Ford et al., 2001, 2002; Mishra et al., 2001, 2002a,b).”
Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses. (2006, neutral): “For experiments monitoring the effects of interfering with CME, time-lapse images of sypHy (512 × 512 pixels) were acquired at 0.5 Hz with 250 ms of exposure at 25% illumination.”