Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity.

By J. O'Brien, Ian A. Wilson, T. Orton, FranÃ§ois Pognan. European Journal of Biochemistry, 2000

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Critical review on biofilm methods (2017, background): “…and highly fluorescent resorufin can be monitored visually, by spectrophotometry or spectrofluorometry (for increased sensitivity); (ii) is less time-consuming (Peeters et al., 2008); (iii) resazurin is not toxic to eukaryotic and prokaryotic cells; and (iv) is inexpensive (O'Brien et al., 2000).”
Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. (2008, background): “This is a blue compound that can be reduced to pink resorufin, which is fluorescent (O'Brien et al., 2000).”
Bright ideas for chemical biology. (2008, methodology): “Resazurin can be reduced to resorufin by biological reducing equivalents and, thus, has been used to assay cell viability (162).”