Targeted Deletion of a High-Affinity GATA-binding Site in the GATA-1 Promoter Leads to Selective Loss of the Eosinophil Lineage In Vivo
By Channing Yu, A. Cantor, Haidi Yang, C. Browne, R. Wells, Y. Fujiwara, Stuart H. Orkin. Journal of Experimental Medicine, 2002
642 citations recorded by the source corpus.
Transcription factor GATA-1 reprograms immature myeloid cells to three different hematopoietic lineages-erythroid cells, megakaryocytes, and eosinophils. GATA-1 is essential for maturation of erythroid and megakaryocytic precursors, as revealed by gene targeting in mice. Here we demonstrate that deletion of a high-affinity GATA-binding site in the GATA-1 promoter, an element presumed to mediate positive autoregulation of GATA-1 expression, leads to selective loss of the eosinophil lineage. These findings suggest that GATA-1 is required for specification of this lineage during hematopoietic development. Mice lacking the ability to produce eosinophils should prove useful in ascertaining the role of eosinophils in a variety of inflammatory or allergic disorders.
Subfields: Epidemiology, Genetics, Physiology
View Paper: https://www.semanticscholar.org/paper/670ea2113b0d4893d816e4fc411cdc696212e774
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Citations in the corpus
- Systemically dispersed innate IL-13–expressing cells in type 2 immunity (2010, background): “IL-4 reporter (4get, KN2) (18, 24), mast cell-deficient Kit (34), and eosinophil-deficient ΔdblGATA (35) mice have been described.”
- Meteorin-like is a hormone that regulates immune-adipose interactions to increase beige fat thermogenesis (2014, methodology): “To determine whether eosinophils are the primary source of IL4/IL13 upon Metrnl treatment, we used mice with a Gata1 promoter mutation that lacks eosinophils (DdblGATA mice) (Yu et al., 2002).”
- Eosinophils: Biological Properties and Role in Health and Disease (2008, background): “…most important for eosinophil lineage specification as revealed by loss of the eosinophil lineage in mice harboring a targeted deletion of the highaffinity GATA-binding site in the GATA-1 promoter (Yu et al. 2002), and based on eosinophil differentiation experiments in vitro (Hirasawa et al. 2002).”
- Innate lymphoid type 2 cells sustain visceral adipose tissue eosinophils and alternatively activated macrophages (2013, background): “…(Mombaerts et al., 1992) or Rag2 (Taconic Farms RAGN12), Rag2 x cdeficient mice (Taconic Farms 4111M), eosinophil deficient dblGATA mice (Yu et al., 2002), IL4/13–deficient mice (McKenzie et al., 1999), Stat6deficient mice (The Jackson Laboratory; Kaplan et al., 1996), IL7Rdeficient…”