A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae.

By V. Rubio, F. Linhares, R. Solano, Ana C. Martín, Joaquín Iglesias, A. Leyva, J. Paz-Ares. Genes & Development, 2001

1,257 citations recorded by the source corpus.

Plants have evolved a number of adaptive responses to cope with growth in conditions of limited phosphate (Pi) supply involving biochemical, metabolic, and developmental changes. We prepared an EMS-mutagenized M(2) population of an Arabidopsis thaliana transgenic line harboring a reporter gene specifically responsive to Pi starvation (AtIPS1::GUS), and screened for mutants altered in Pi starvation regulation. One of the mutants, phr1 (phosphate starvation response 1), displayed reduced response of AtIPS1::GUS to Pi starvation, and also had a broad range of Pi starvation responses impaired, including the responsiveness of various other Pi starvation-induced genes and metabolic responses, such as the increase in anthocyanin accumulation. PHR1 was positionally cloned and shown be related to the PHOSPHORUS STARVATION RESPONSE 1 (PSR1) gene from Chlamydomonas reinhardtii. A GFP::PHR1 protein fusion was localized in the nucleus independently of Pi status, as is the case for PSR1. PHR1 is expressed in Pi sufficient conditions and, in contrast to PSR1, is only weakly responsive to Pi starvation. PHR1, PSR1, and other members of the protein family share a MYB domain and a predicted coiled-coil (CC) domain, defining a subtype within the MYB superfamily, the MYB-CC family. Therefore, PHR1 was found to bind as a dimer to an imperfect palindromic sequence. PHR1-binding sequences are present in the promoter of Pi starvation-responsive structural genes, indicating that this protein acts downstream in the Pi starvation signaling pathway.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/6549254241ae12ed7dc9364c06a8f5263b9fd149

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Citations in the corpus

Phosphate transport in plants (2004, background): “TATNC has been identified in the promoter regions of some phosphate-responsive genes, including some Pi transporters (Rubio et al., 2001).”
Feeding hungry plants: The role of purple acid phosphatases in phosphate nutrition (2010, background): “The transcription factors PHR1, WRKY75, and ZAT6 have been implicated in the control of Pistarvation inducible purple acid phosphatase expression [72–74],”
Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. (2003, background): “, 1999) and Arabidopsis (Rubio et al. 2001) as well as soybean (Glycine max) P response genes (Tang et al.”
The Role of PHT1 Family Transporters in the Acquisition and Redistribution of Phosphorus in Plants (2019, background): “MYB and other MYB TFs localized in the nucleus bind to the P1BS or P1BS-like domain in PHT1 gene promoters and up-regulate the expression of PHT1 genes (Rubio et al., 2001; Sch€ unmann et al., 2004a; Sch€unmann et al., 2004b; Bustos et al., 2010; Meina et al., 2015).”
Plant abiotic stress response and nutrient use efficiency (2020, background): “control the expression of phosphate starvation-induced (PSI) genes (Rubio et al., 2001; Zhou et al., 2008).”
Phosphate Acquisition (2004, background): “Genes coding for MYB transcription factors in both algae (PSR1) and higher plants (PHR1) appear to regulate the expression of many Pi-starvation-induced genes, including some Pi transporters (Rubio et al., 2001; Wykoff et al., 1999).”
WRKY75 Transcription Factor Is a Modulator of Phosphate Acquisition and Root Development in Arabidopsis1[C][W][OA] (2007, background): “…www.plantphysiol.org/cgi/doi/10.1104/pp.106.093971 Plant Physiology, April 2007, Vol. 143, pp. 1789–1801, www.plantphysiol.org 2007 American Society of Plant Biologists 1789 factor PHR1 was first reported to play a regulatory role in Pi starvation responses in Arabidopsis (Rubio et al., 2001).”
Phosphorus Plays Key Roles in Regulating Plants’ Physiological Responses to Abiotic Stresses (2023, background): “One of these groups is responsible for sensing low P signals, encompassing both local phosphate sensing in the primary root of Arabidopsis thaliana [60] and long-distance phosphate signaling mediated by transcription factors such as PHR1 and PHL1 [61].”
Root structure and functioning for efficient acquisition of phosphorus: Matching morphological and physiological traits. (2006, background): “The promoters of the genes encoding the high-affinity transporter and the acid phosphatase contain a short sequence that is identical to the binding site for a transcription activator for P-deficiency-induced genes in A. thaliana (Rubio et al., 2001).”
Uncovering Small RNA-Mediated Responses to Phosphate Deficiency in Arabidopsis by Deep Sequencing1[W][OA] (2009, background): “…on the PHOSPHATE STARVATION RESPONSE1 (PHR1) MYB transcription factor, which activates a group of Pi starvation-induced genes, includingMIR399s (Rubio et al., 2001; Bari et al., 2006), we analyzed the existence of P1BS cis-element, the biding site of PHR1, within the 2-kb sequences upstream of…”
Regulation of Phosphate Homeostasis by MicroRNA in Arabidopsis[W] (2005, background): “PHOSPHATE STARVATION RESPONSE1, a MYB transcription factor, is the only well-characterized regulator involved in the upregulation of a specific group of P-responsive genes through a GNATATNC cis element (Rubio et al., 2001; Franco-Zorrilla et al., 2004).”