WRKY75 Transcription Factor Is a Modulator of Phosphate Acquisition and Root Development in Arabidopsis1[C][W][OA]

By B. N. Devaiah, Athikkattuvalasu S. Karthikeyan, K. Raghothama. Plant Physiology, 2007

668 citations recorded by the source corpus.

Phosphate (Pi) deficiency limits plant growth and development, resulting in adaptive stress responses. Among the molecular determinants of Pi stress responses, transcription factors play a critical role in regulating adaptive mechanisms. WRKY75 is one of several transcription factors induced during Pi deprivation. In this study, we evaluated the role of the WRKY75 transcription factor in regulating Pi starvation responses in Arabidopsis (Arabidopsis thaliana). WRKY75 was found to be nuclear localized and induced differentially in the plant during Pi deficiency. Suppression of WRKY75 expression through RNAi silencing resulted in early accumulation of anthocyanin, indicating that the RNAi plants were more susceptible to Pi stress. Further analysis revealed that the expression of several genes involved in Pi starvation responses, including phosphatases, Mt4/TPS1-like genes, and high-affinity Pi transporters, was decreased when WRKY75 was suppressed. Consequently, Pi uptake of the mutant plant was also decreased during Pi starvation. In addition, when WRKY75 expression was suppressed, lateral root length and number, as well as root hair number, were significantly increased. However, changes in the root architecture were obvious under both Pi-sufficient and Pi-deficient conditions. This indicates that the regulatory effect of WRKY75 on root architecture could be independent of the Pi status of the plant. Together, these results suggest that WRKY75 is a modulator of Pi starvation responses as well as root development. WRKY75 is the first member of the WRKY transcription factor family reported to be involved in regulating a nutrient starvation response and root development.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/aadd66cd3ffe7fed0f4f77a81e0072de83fdd372

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Citations in the corpus

• The roles of membrane transporters in arsenic uptake, translocation and detoxification in plants (2020, background): “In Arabidopsis, four WRKY transcription factors (WRKY6, WRKY42, WRKY45 and WRKY75) have been implicated in the phosphate starvation signaling (Chen et al., 2009; Devaiah et al., 2007; Su et al., 2015; Wang et al., 2014).”
• The Role of PHT1 Family Transporters in the Acquisition and Redistribution of Phosphorus in Plants (2019, background): “thaliana WRKY75 and WRKY45 are up-regulated and act as positive regulators for AtPHT1;1, whereas WRKY6 and WRKY42 are down-regulated at the transcriptional level and/or protein level in LPC (Devaiah et al., 2007a; Chen et al., 2009; Wang et al., 2014a; Su et al., 2015).”
• WRKY transcription factors (2014, background): “Suppression of WRKY75 expression through RNAi silencing resulted in early accumulation of anthocyanin, indicating that the RNAi plants were more susceptible to Pi stress.(85) WRKY75 is a positive regulator of several Pi starvationinduced genes including phosphatase, Mt4/TPS1-like genes and high affi”
• A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis (2010, background): “In addition to PHR1, members of bHLH, WRKY, Zinc finger and R2R3 MYB families of TF are involved in the control of Pi starvation responses, although their exact positions in the signalling pathway have not been established [13–17].”
• Phosphorus Plays Key Roles in Regulating Plants’ Physiological Responses to Abiotic Stresses (2023, background): “Additionally, other transcription factors, including WRKY74, WRKY75, MYB2, MYB4 and ARF16, have been associated with the PSR pathway [92,93].”
• The WRKY Transcription Factor Family in Model Plants and Crops (2017, background): “In the AtWRKY75 knockout mutant, the number and length of the root hairs show an increase compared with the wildtype, suggesting that AtWRKY75 is a negative regulator of root hair development (Devaiah et al., 2007).”
• Heterologous expression of OsWRKY23 gene enhances pathogen defense and dark-induced leaf senescence in Arabidopsis (2009, background): “…of sequencespecific DNA binding transcription factors of plant defense genes have been identified, including WRKY factors which have been implicated in the regulation of pathogen defense, abiotic stress response and development process (Yu et al. 2001; Miao et al. 2004; Devaiah et al. 2007).”
• Feeding hungry plants: The role of purple acid phosphatases in phosphate nutrition (2010, background): “The transcription factors PHR1, WRKY75, and ZAT6 have been implicated in the control of Pistarvation inducible purple acid phosphatase expression [72–74],”
• Signaling network in sensing phosphate availability in plants. (2011, background): “RNAi transgenic plants of WRKY75 reduced the upregulation of several PSI genes but increased the growth of lateral roots and root hairs independently of Pi status (25).”
• The role of WRKY transcription factors in plant abiotic stresses. (2012, background): “Transcript levels of both AtWRKY6 and AtWRKY75 are enhanced during phosphate deprivation [35,36].”