Evaluation of Bacillus subtilis as a probiotic to Indian major carp Labeo rohita (Ham.)

By Rajesh Kumar, S. Mukherjee, K. Prasad, A. K. Pal. 2006

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Bacillus subtilis, a Gram-positive, aerobic, endosporeforming bacterium, was evaluated for its probiotic potential in Indian major carp, Labeo rohita. Labeo rohita (15 � 2 g) were fed a feed containing B. subtilis in three concentrations for 2 weeks, e.g., 0.5 (T2), 1.0 (T3) and 1.5 (T4) � 10 7 CFU g � 1 feed. The control group (T1) was fed feed without B. subtilis for the same period. Haematological and serum parameters were monitored at weekly intervals. The response variables were total erythrocyte count, total leucocyte count (TLC), haemoglobin, total protein, albumin, globulin, albumin^globulin ratio, alkaline phosphatase activity, alanine aminotransferase activity and aspartate aminotransferase activity. Fish were challenged intraperitoneally with a virulent strain of Aeromonas hydrophila after 2 weeks of feeding to the treatment groups and positive control group, while the negative control group was challenged with phosphate-buiered saline only. Clinical signs and symptoms, and mortality/survival percentage were noted in each group. The haematological and serum parameters were monitored each week and during post challenge on the third and tenth day. The B. subtilis-treated ¢sh (T4,1.5 � 10 7 CFU g � 1 feed) showed maximum per cent survival (87.50%), weight gain (35.5%), TLCs (3.23 � 10 4 cells mm � 3 ), haemoglobin content (7.4 g%), total protein (2.37 g dL � 1 ) and globulin content (1.28 g dL � 1 )d uring the pre-challenge. Enzymes showed higher activities during post challenge (Po0.05). The result suggests that B. subtilis can be used eiectively as a commercial product for use in aquaculture.

Subfields: Aquatic Science, Immunology

View Paper: https://www.semanticscholar.org/paper/64dcf1e90878dcc79a72c36833b377d5baefee39

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Citations in the corpus

Application of Probiotics in Shrimp Aquaculture: Importance, Mechanisms of Action, and Methods of Administration (2016, background): “It is considered as etiological agent for more than a few diseases including emaciation, hemorrhagic septicemia, asymptomatic septicemia, ulcerative infection, tail rot, and fin rot (Rahman et al., 2001; Kumar et al., 2006).”
Review: Developments in the use of probiotics for disease control in aquaculture (2014, background): “The literature abounds with evidence for the benefit of using the so-called probiotics, specifically in terms of growth benefits (Boonthai et al., 2011; Brunt and Austin, 2005; El-Haroun et al., 2006; Kumar et al., 2006; Macey and Coyne, 2005; Silva et al., 2013) [although retardation of growth may ”
Probiotic effect of Bacillus NL110 and Vibrio NE17 on the survival, growth performance and immune response of Macrobrachium rosenbergii (de Man) (2010, background): “…in inhibition of pathogenic bacteria (Austin, Stuckey, Robertson, E¡endi & Gri⁄th 1995; Gatesoupe 1997; Huys et al. 2001), enhancing enzyme activity (Kumar et al. 2006; Ghosh, Sinha & Sahu 2008), better feed conversion, fecundity and fry production (Wang & Xu 2006; Ghosh, Sinha & Sahu 2007) as…”
Probiotics in aquaculture: The need, principles and mechanisms of action and screening processes (2008, background): “…et al. (2006) Goldfish Dead cells of A. hydrophila A. salmonicida In vivo Irianto et al., 2003 Indian major carp B. subtilis A. hydrophila In vivo Kumar et al. (2006) Nile tilapia Str. faecium, Lactobacillus acidophilus, Sacc. cerevisiae Growth study In vivo Lara-Flores et al. (2003) Pollack…”
Use of different Saccharomyces cerevisiae biotic forms as immune-modulator and growth promoter for Oreochromis niloticus challenged with some fish pathogens (2013, background): “Thus, the brewer yeast is a vital source of vitamin B complex as well as other haemotonic ingredients utilized in the process of haematopoisis [49,50].”
Genus bacillus, promising probiotics in aquaculture: Aquatic animal origin, bio-active components, bioremediation and efficacy in fish and shellfish (2019, background): “The first study using B. subtilis as a fish growth promoter was by Kumar et al. (2006), who fed different levels (0.5, 1.0, and 1.5 107 CFU g 1) of B. subtilis to rohu for 15 days and observed significant increase in weight gain.”
Evaluation of Bacillusamyloliquefaciens on the growth performance, intestinal morphology, hematology and body composition of Nile tilapia, Oreochromis niloticus (2014, result): “Results of this study, together with those reported in literature (Kumar et al. 2006; Nayak et al. 2007) confirm the positive effect of probiotics in increasing serum protein and globulin levels.”