Resolution of phase ambiguity in macromolecular crystallography.

By B. C. Wang. 1985

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Citations in the corpus

Using SAD data in Phaser (2011, neutral): “The introduction of density-modification methods, such as solvent flattening (Wang, 1985), made it possible to resolve the phase ambiguity without adding information from multiple wavelengths or multiple heavy-atom derivatives.”
Recent developments in classical density modification (2010, unknown): “(i) Solvent flattening (Wang, 1985).”
Anomalous signal indicators in protein crystallography. (2005, methodology): “This limit, known as Wang’s limit, originates from an example from error-free data (Wang, 1985).”
Recent developments in phasing and structure refinement for macromolecular crystallography (2009, background): “Often these initial maps are very noisy, but powerful density modification techniques can be used to greatly improve their quality [27–29].”
Direct-method SAD phasing of proteins enhanced by the use of intrinsic bimodal phase distributions in the subsequent phase-improvement process. (2009, methodology): “It is essential to improve the initial phases (Sha et al., 1995) by using some kind of phase-improvement procedure, in particular the solventflattening technique (Wang, 1985).”
Regulation of the transcriptional activator NtrC1: structural studies of the regulatory and AAA+ ATPase domains. (2003, methodology): “Using partial models, two separate NCS operators for both regulatory and central ATPase domains were calculated and two domain NCS-averaging was performed using DM (Wang 1985).”
Matrix methods for solving protein substructures of chlorine and sulfur from anomalous data. (2001, methodology): “Wang discussed the structure determination of a small protein (12 kDa) using simulated data and the signal from two sulfurs (Wang, 1985).”
Bringing diffuse X-ray scattering into focus (2017, background): “5 Å range, which is where the diffuse intensity was measured? How does the improvement in the PSII map compare to what would be obtained by using randomized intensities, due to the free lunch effect [57] and solvent flattening [58]? How would the R-factors in the extended resolution range reported i”
A modified ACORN to solve protein structures at resolutions of 1.7 A or better. (2005, background): “(iii) A locally averaged map over a sphere of radius 2 Å is calculated for a map with coefficients W(h)|Fo(h)| for the observed reflections to find an envelope (Wang, 1981, 1985).”
Automated map sharpening by maximization of detail and connectivity (2018, methodology): “The volume occupied by the molecule is identified using tools developed for identification of the region occupied by the macromolecule in crystallographic maps (Wang, 1985, Terwilliger, 1999).”
Automated structure solution, density modification and model building. (2002, methodology): “…density modification Density modi®cation is a well established and very powerful method for improving the quality of electron-density maps (Rossmann, 1972; Bricogne, 1976; Wang, 1985; Xiang et al., 1993; Cowtan & Main, 1993; SzoÈ ke, 1993; Abrahams & Leslie, 1996; van der Plas & Millane, 2000).”
Structure of the insecticidal bacterial δ-endotoxin Cry3Bb1 of Bacillus thuringiensis (2001, methodology): “Solvent-¯attened data were prepared using the program of Wang (1985).”
Can I solve my structure by SAD phasing? Anomalous signal in SAD phasing (2016, methodology): “A measure of signal in anomalous data that can be estimated in advance of carrying out an experiment is the Bijvoet ratio h|F + F |i/hFi (Hendrickson & Teeter, 1981; Wang, 1985).”
Introduction to phasing (2010, methodology): “Increasing numbers of protein structures are now being phased using only a single set of diffraction data by the singlewavelength anomalous dispersion/diffraction (SAD) method (Wang, 1985).”