Application of Denaturing Gradient Gel Electrophoresis (DGGE) To Study the Diversity of Marine Picoeukaryotic Assemblages and Comparison of DGGE with Other Molecular Techniques

By B. Díez, C. Pedrós-Alió, T. Marsh, Ramon Massana. Applied and Environmental Microbiology, 2001

595 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/647406b2f56f37c0e8f9fccfbf249259e343fabb

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Citations in the corpus

Composition and temporal variability of picoeukaryote communities at a coastal site of the English Channel from 18S rDNA sequences (2004, methodology): “Surprisingly, Dı́ez et al. (2001b) did not find any Cryptophyceae sequences in North Atlantic and Antarctic waters, but only in the Mediterranean Sea.”
Dormancy contributes to the maintenance of microbial diversity (2010, methodology): “Terminal restriction fragment length polymorphism (T-RFLP) was conducted on both DNA and cDNA using general 16S [8F and 533R (51)] and 18S [Euk1Af and Euk516r (52)] rRNA primers.”
Lotic biofilm community structure and pesticide tolerance along a contamination gradient in a vineyard area (2007, methodology): “…deionized formamide were used as denaturing agents, and the gel gradient varied from from 40% (top) to 80% (bottom) for PCR-amplified 16S rRNA gene fragments (Schauer et al. 2000, Dorigo et al. 2006), and from 40% (top) to 65% (bottom) for PCR-amplified 18S rRNA gene fragments (Díez et al. 2001a).”
Seasonal changes in the taxonomic composition of bacterioplankton in a coastal oligotrophic system (2003, background): “Extensive background information exists about this site (Duarte et al. 1999).”
Quality analysis of commercial Chlorella products used as dietary supplement in human nutrition (2010, methodology): “…rDNA fragment Cya 359r Nübel et al. 1997 Amplification of 16S rDNA Cya 781f Nübel et al. 1997 Amplification of 16S rDNA Table 1 Oligonucleotide primers for amplification of 16S rDNA and 18S rDNA a PCR primer with GC-clamp: CGCCCGGGGCGCGCCCCG GGCGGGGCGGGGGCACGG GG GG (after Diez et al. 2001) (Fig.”
DGGE coupled with ribosomal DNA gene phylogenies reveal uncharacterized fungal phylotypes (2006, methodology): “123 electrophoresis (DGGE) is a technique that has effectively been used to estimate the diversity of prokaryotes and eukaryotes in natural samples (Anderson et al., 2004; Countway et al., 2005; Dar et al., 2005; Díez et al., 2001;Jeewon and Hyde, 2006).”
Effects of straw amendment and moisture on microbial communities in Chinese fluvo-aquic soil (2014, methodology): “To produce eukaryotic amplicon libraries for 18S rRNA gene pyrosequencing, partial eukaryotic 18S rRNA gene fragments were amplified using the primer set Euk1A (5′-CTGG TTGATCCTGCCAG-3′)/Euk516r (5′-ACCAGACTTGCC CTCC-3′) (Diez et al. 2001).”
Significance of predation by protists in aquatic microbial food webs (2004, background): “Recent analysis of DNA extracted from picoeukaryotic cells in surface seawater has yielded evidence for abundant diversity, with a number of gene clones that may be affiliated with heterotrophic protists (Diez et al. 2001a,b; Moon-van der Staay et al. 2001).”
Genetic diversity of eukaryotic ultraphytoplankton in the Gulf of Naples during an annual cycle (2007, background): “Recent genetic studies have highlighted the taxonomic diversity of these small cells (Rappé et al. 1995, Potter et al. 1997, Díez et al. 2001a,b, Moon-van der Staay et al. 2001, Medlin et al. 2006), including revealing new classes and novel lineages (Guillou et al. 1999, Massana et al. 2002).”