Seasonal changes in the taxonomic composition of bacterioplankton in a coastal oligotrophic system

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Influence of slight differences in environmental conditions (light, hydrodynamics) on the structure and function of periphyton (2009, methodology): “PCR amplification of the bacterial 16S rRNA gene fragment was done with 30 ng of template DNA, and the primers 341f, to which a GC-rich fragment was attached (Muyzer and Smalla 1998), and 907rM (Schauer et al. 2003), which yielded a fragment of *590 bp.”
Seasonal changes in bacterioplankton nutrient limitation and their effects on bacterial community composition in the NW Mediterranean Sea (2006, methodology): “Filters were immediately frozen at –70°C in sucrose buffer until further processing; community DNA was obtained using a standard phenol-extraction protocol (Schauer et al. 2003).”
Spatial and temporal scales of variation in bacterioplankton community structure in the NW Mediterranean Sea (2005, background): “Marine bacterial communities are also influenced by temporal changes that occur at different time scales, from years or seasons (Lee & Furhman 1991, Murray et al. 1998, Riemann et al. 1999, Schauer et al. 2000, 2003) to weeks or days (Acinas et al. 1997, Fandino et al. 1998, 2001).”