Functional annotation of native enhancers with a Cas9 -histone demethylase fusion
By Nicola A. Kearns, Hannah Pham, B. Tabak, Ryan M. J. Genga, Noah J. Silverstein, Manuel Garber, René Maehr. Nature Methods, 2015
642 citations recorded by the source corpus.
Understanding of mammalian enhancers is limited by the lack of a technology to rapidly and thoroughly test the cell type–specific function. Here, we use a nuclease-deficient Cas9 (dCas9)–histone demethylase fusion to functionally characterize previously described and new enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/6397a20b5b2b9a4fa4f62d80da9a79ec8bae98ab
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Citations in the corpus
- Highly Specific Epigenome Editing by CRISPR/Cas9 Repressors for Silencing of Distal Regulatory Elements (2015, positive): “44). dCas9-KRAB targeted to endogenous promoters can induce repressive histone methylation 9 .”
- Crystal structure of Cpf1 in complex with guide RNA and target DNA (2016, background): “, 2015) to targeted modulation of histone marks (Hilton et al., 2015; Kearns et al., 2015).”
- CRISPR/Cas9 in Genome Editing and Beyond. (2016, methodology): “The studies (38, 39, 203) described above have demonstrated that dCas9 fusion proteins can act as sequence-specific, synthetic epigenome modifiers, which not only change local epigenetic status but also change the gene expression of relevant genes.”
- Ever-changing landscapes: transcriptional enhancers in development and evolution (2016, background): “Conversely, RNA-guided recruitment of the H3K4me1/2 demethylase LSD1 is sufficient to repress enhancer activity and gene transcription (Kearns et al., 2015).”
- Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems (2016, methodology): “The dCas9 has been used for transcriptional activation and repression (135–138), localizing fluorescent protein labels (139), and recruiting histone-modifying enzymes (140, 141).”