Transcriptional pulsing of a developmental gene.

By Jonathan R. Chubb, Tatjana Trcek, S. Shenoy, R. Singer. Current Biology, 2006

734 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/6256b036b315fdfcf0c54ea3445102012f9b9f93

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In Molecular Biology

Citations in the corpus

Nuclear organization of the genome and the potential for gene regulation (2007, background): “Studies done in live cells have shown that individual genes are transcribed in bursts or pulses of productio”
In vivo dynamics of RNA polymerase II transcription (2007, background): “If the gene were highly expressed (that is, if its transcription were initiated frequently) this could result in 'pulsing' of transcriptio”
Stochastic mRNA Synthesis in Mammalian Cells (2006, background): “[23] showed through temporal measurements of active transcription sites that genes do indeed undergo random transitions between transcriptionally active and inactive states, providing a powerful corroboration of our model.”
Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells (2009, background): “Transcription of most 'active' genes is not continuous but occurs in pulses of activit”
Stochastic gene expression and its consequences (2008, methodology): “For instance, utilizing the MS2-GFP method of mRNA detection (Beach et al., 1999; Bertrand et al., 1998), Chubb et al. (2006) showed that a developmental gene in Dictyostelium discoideum is transcribed in a pulsatile fashion, directly demonstrating the burst hypothesis by watching mRNAs accumulate…”
Single-Cell and Single-Molecule Analysis of Gene Expression Regulation (2016, background): “Conversely, the social amoeba Dictyostelium is more likely to re-express an endogenous developmental gene than initiate new expression (the probability to switch from OFF state to ON state is higher) (33).”
Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq. (2011, positive): “Most of the variation may be intrinsic, caused by burst-like stochastic activation of transcription, where brief episodes of mRNA synthesis lasting a few minutes are separated by periods of transcriptional silence of similar duration (Chubb et al. 2006).”
Correlation of mRNA and protein in complex biological samples (2009, positive): “This ability to measure has provided important details of how transcription is carried out in pulses [10–12].”
Slingshot: cell lineage and pseudotime inference for single-cell transcriptomics (2017, background): “In addition to the host of biological and technical sources of variation that can affect any (bulk) RNA-Seq experiment, single-cell data may contain effects from transcriptional bursting [23, 24] and drop-out [25].”
Dynamic regulation of nucleosome positioning in the human genome. (2008, positive): “These results are consistent with the previous observation that active transcription is detected at only a fraction of cells at any one moment (Chubb et al., 2006).”
Analytical distributions for stochastic gene expression (2008, background): “Such bursts of gene expression have been measured in bacteria and eukaryotes [12, 13, 14, 28, 29].”